Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Formation of Complex Ions03:45

Formation of Complex Ions

23.7K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
23.7K
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism01:37

1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism

3.8K
Nitrous acid is a relatively weak and unstable acid prepared in situ by the reaction of sodium nitrite and cold, dilute hydrochloric acid. In an acidic solution, the nitrous acid undergoes protonation when it loses water to form a nitrosonium ion—an electrophile. Nitrous acid reacts with primary amines to give diazonium salts. The reaction is called diazotization of primary amines.
3.8K
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview01:26

1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview

3.3K
Nitrous acid and nitric acids are two types of acids containing nitrogen, among which nitrous acid is weaker than nitric acid. Nitrous acid with a pKa value of 3.37 ionizes in water to give a nitrite ion and the hydronium ion.
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...
3.3K
2° Amines to N-Nitrosamines: Reaction with NaNO201:20

2° Amines to N-Nitrosamines: Reaction with NaNO2

4.3K
Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
4.3K
Nitrosation of Enols01:19

Nitrosation of Enols

2.9K
The nitrosation reaction is one of the methods of preparing 1,2-diketones. The enol tautomer of the starting ketone reacts with sodium nitrite in hydrochloric acid, generating the 1,2-diketone after hydrolysis.
2.9K
Rate-Determining Steps03:08

Rate-Determining Steps

32.5K
Relating Reaction Mechanisms
In a multistep reaction mechanism, one of the elementary steps progresses significantly slower than the others. This slowest step is called the rate-limiting step (or rate-determining step). A reaction cannot proceed faster than its slowest step, and hence, the rate-determining step limits the overall reaction rate.
The concept of rate-determining step can be understood from the analogy of a 4-lane freeway with a short-stretch of traffic-bottleneck caused due to...
32.5K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Copper(II/III) Redox Couple Enables C─H Methylation via a Radical Mechanism Analogous to SAM Enzymes.

Angewandte Chemie (International ed. in English)·2026
Same author

Thermodynamics-Informed Machine Learning of Organic Electrode Material Solubility in Nonaqueous Electrolytes.

The journal of physical chemistry. B·2026
Same author

Synthesis, Characterization, and Evaluation of the Cobalt-Carbon Bond Strength of (PNNP)Co<sup>III</sup>-R Complexes.

Organometallics·2026
Same author

Role of the Character of the Excited State of Singly Reduced Rh<sub>2</sub>(II,II) Intermediates on Photocatalytic Activity.

Journal of the American Chemical Society·2026
Same author

Mechanistic Continuum from Stepwise to Concerted Proton-Coupled Electron Transfer Pathways at a Synthetic Tricopper Cluster.

Journal of the American Chemical Society·2026
Same author

Structure-Activity Relationship Studies toward the Optimization of First-In-Class Selective Small Molecule Agonists of the GPCR Relaxin/Insulin-like Family Peptide Receptor 2.

Journal of medicinal chemistry·2026

関連する実験動画

Updated: Jul 14, 2025

Preparation of 6-aminocyclohepta-2,4-dien-1-one Derivatives via Tricarbonyltroponeiron
07:56

Preparation of 6-aminocyclohepta-2,4-dien-1-one Derivatives via Tricarbonyltroponeiron

Published on: August 12, 2019

8.0K

ダイアイロン・ディニトロシル・コンプレックスにおけるニートリート形成

Anna L Poptic1, Jeffrey K Klinger1, Samantha L Carter1

  • 1Department of Chemistry & Biochemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio43210, United States.

Journal of the American Chemical Society
|October 10, 2023
PubMed
まとめ
この要約は機械生成です。

この研究は,細菌酵素の酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化酸化 モデルコンプレックスはNOをナトリートに変換し,細菌の免疫回避メカニズムに洞察を与えます.

さらに関連する動画

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.0K
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

22.6K

関連する実験動画

Last Updated: Jul 14, 2025

Preparation of 6-aminocyclohepta-2,4-dien-1-one Derivatives via Tricarbonyltroponeiron
07:56

Preparation of 6-aminocyclohepta-2,4-dien-1-one Derivatives via Tricarbonyltroponeiron

Published on: August 12, 2019

8.0K
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.0K
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

22.6K

科学分野:

  • バイオ有機化学
  • 酵素ミミクリ
  • バクテリア病原性

背景:

  • 病原菌は 哺乳類の免疫分子である 酸化窒素 (NO) を解毒するために 鉄を含む酵素を利用します
  • フラボアイロン酸化窒素還元酵素 (FNORs) とヘメリトリンのようなタンパク質 (HLPs) は,NO代謝に関与する重要な細菌酵素である.
  • FNORはNOを減らし,Mka-HLPのような特定のHLPはNOを窒素化し,合成モデルではまだ複製されていません.

研究 の 目的:

  • 電子が少ないMka-HLPの活性部位をモデル化した新しい二鉄複合体を合成する.
  • この合成複合体の窒素酸化反応性を調査する.
  • Mka-HLPで観察されたNO酸化酵素の活性を再現する最初の合成モデルを提供すること.

主な方法:

  • Mka-HLPの電子が少ない調整環境を模倣するように設計された新しい二鉄複合体の合成.
  • デフェルアスの前駆体と酸化窒素 (NO) の反応により,ダイアイロン・ディニトロシルとモノニトロシル種が形成される.
  • NO酸化に焦点を当てた,結果のニトロシル種の分離,特徴付け,機能試験.

主要な成果:

  • ダイアイロン・ディニトロシル種 (FeNO2) とモノニトロシル・ディアイロン種 (FeNO) が形成され,特徴づけられた.
  • ダイアイロン・ディニトロシル (FeNO2) の酸化により,高効率の71%のニートリートが得られた.
  • これは,Mka-HLPのNO酸化酵素の活性を初めて成功裏に合成したことを示しています.

結論:

  • 合成された二鉄複合体は,Mka-HLPのNO酸化作用を効果的にモデル化している.
  • この研究は,このNO酸化プロセスにおける中介物としての{FeNO}2/{FeNO}種の関与を示唆している.
  • この研究は,NOの解毒と免疫回避のためのバクテリアの戦略に貴重な洞察を提供します.