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関連する概念動画

Transcription Factors02:16

Transcription Factors

76.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
76.7K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.5K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.5K
General Transcription Factors01:30

General Transcription Factors

5.5K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.5K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

9.4K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.4K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.5K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.5K
Master Transcription Regulators02:23

Master Transcription Regulators

7.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.0K

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Updated: Sep 9, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

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複数の重複する結合部位が転写因子占有率を決定する

Shubham Khetan1, Brent S Carroll1, Martha L Bulyk2,3

  • 1Division of Genetics, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.

Nature
|September 3, 2025
PubMed
まとめ
この要約は機械生成です。

私たちはPADIT-seqを開発し,トランスクリプション因子 (TF) の新しい低親和性DNA結合部位を発見しました. これは重複する結合部位が 集団的に遺伝子発現を制御し 人間の特徴や病気に 影響を及ぼすことを示しています

さらに関連する動画

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis
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Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

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Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
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関連する実験動画

Last Updated: Sep 9, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

8.9K
Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis
12:29

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

Published on: April 16, 2018

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Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA
07:05

Single-Molecule Imaging of EWS-FLI1 Condensates Assembling on DNA

Published on: September 8, 2021

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科学分野:

  • ゲノミクス
  • 分子生物学
  • バイオ情報学

背景:

  • 転写因子 (TF) は,配列特異のDNA相互作用を通じて遺伝子発現を調節する.
  • 遺伝子調節に不可欠な低親和性TF結合部位を特定するために,既存の高スループットメソッドは苦労しています.
  • 低親和性サイトは,精密な空間時間的遺伝子発現制御における役割としてますます認識されています.

研究 の 目的:

  • TF DNA結合偏好を総合的に測定するための新しい方法を開発する.
  • 以前に検出されなかった低親和TF結合部位を特定する.
  • TF結合と遺伝子調節と疾患におけるその役割に関する新しいモデルを提案する.

主な方法:

  • タンパク質とDNAの親和性を in vitro トランスクリプションとRNAシーケンシング (PADIT-seq) により開発する.
  • すべての10塩基対DNA配列で6つのTFの結合偏好の包括的な測定.
  • TF結合部位の重複とそのゲノム占有率への影響の分析

主要な成果:

  • PADIT-seqは,TFsのための新しい,より低いアフィニティのDNA結合部位を成功裏に検出しました.
  • 高 afinity サイトを横切る核酸は,in vivo TF 結合を調節する低 afinity サイトを重複させます.
  • 複数の重なり合っている場所の合計に基づいたTFの結合モデルが提案されました.

結論:

  • TF結合は,単一の高親和性サイトではなく,複数の重複する結合サイトの集合的効果によって決定されます.
  • 相互に重なり合う結合モデルは,TFの競争と,類似のTFによる結合場所の使用の差を説明する.
  • このモデルは,非コーディング変異の影響を再定義し,遺伝子の発現,人間の特徴,病気に影響を与えるために複数のサイトをどのように変化させるかを示しています.