Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

ABC Transporters: Importer01:27

ABC Transporters: Importer

3.4K
ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
3.4K
ABC Transporters: Exporter01:31

ABC Transporters: Exporter

6.3K
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
6.3K
Transduction01:16

Transduction

1.2K
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
1.2K
Bacterial Transformation01:33

Bacterial Transformation

59.3K
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
59.3K
Antibiotic Selection00:57

Antibiotic Selection

59.4K
Overview
59.4K
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

513
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
513

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Metronidazole and ether derivatives target Helicobacter pylori via simultaneous stress induction and inhibition.

Nature microbiology·2026
Same author

Single-molecule identification of full-length proteins with single-amino-acid resolution using nanopores.

bioRxiv : the preprint server for biology·2026
Same author

Structure-Based Design of Pan-Selective Peptide Epoxyketones for the Three Human Immunoproteasome Active Sites.

Journal of medicinal chemistry·2026
Same author

Structural Mimics of Hydrocarbon Intermediates Reveal Counterclockwise Cyclization Pathways in the Sesquiterpene Synthases TmS and NcECS.

Journal of the American Chemical Society·2025
Same author

Tellurophene-Tagged Carfilzomib Enables Single-Cell Mass Cytometric Mapping of Proteasome Activity.

ACS chemical biology·2025
Same author

A Luminescence-Based Screening Platform for Lanthanide-Binding Peptides and Proteins.

ACS chemical biology·2025

関連する実験動画

Updated: Jan 15, 2026

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

1.0K

遺伝子コードの拡張のためにバクテリアのABCトランスポーターをハイジャック

Tarun Iype1, Maximilian Fottner1, Paul Böhm1

  • 1Department of Chemistry and Applied Biosciences (D-CHAB), ETH Zurich, Zurich, Switzerland.

Nature
|October 15, 2025
PubMed
まとめ

研究者は,非正規のアミノ酸 (ncAAs) の細胞吸収を改善するために,細菌のトランスポーターを設計し,遺伝子コードの拡張とタンパク質の機能化の重要なボトルネックを克服しました.

さらに関連する動画

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.1K
Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
09:00

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

12.3K

関連する実験動画

Last Updated: Jan 15, 2026

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

1.0K
Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.1K
Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
09:00

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

12.3K

科学分野:

  • 生物化学
  • 分子生物学
  • 合成生物学

背景:

  • 非法定アミノ酸 (ncAAs) のサイト固有の組み込みは,タンパク質の機能性を拡張しますが,組み込み効率が低いため制限されます.
  • ncAAsの細胞吸収が悪いことは,効率的な遺伝子コードの拡張を妨げている主要な障害です.

研究 の 目的:

  • 細胞の不十分な吸収のボトルネックを克服し,ncaaの組み込みを強化する.
  • タンパク質に様々な化学的構成要素を効率的かつプログラム可能な輸入のための新しい戦略を開発する.

主な方法:

  • バクテリアのATP結合カセット (ABC) トランスポーターをハイジャックし,nCAAsを積極的に輸入する.
  • 簡単に合成可能なイソペプチド結合トリペプチドが細胞内でのncaaに処理されます.
  • 耐火性ncAAsのトランスポーターシステムを設計するための高通量指向進化プラットフォームを開発しました.

主要な成果:

  • 以前はアクセスできないncAAsの効率的なエンコーディングを達成し,バイオオルトゴン部分,クロスリンカー,および翻訳後の改変でタンパク質の装飾を可能にしました.
  • 野生型効率で単一および複数サイトNCAA組み込みのためのカスタマイズされたEscherichia coli株.
  • 2つの異なるncAAsの効率的な共同輸送と二重組み込みのための適応されたトリペプチド・スキャフォールド.

結論:

  • 遺伝子コードの拡張を 強化する強力な戦略です 遺伝子コードの拡張を 強化する強力な戦略です
  • 開発されたアプローチは,化学的に多様な構成要素の広範なプログラム可能な輸入を可能にします.
  • この研究は,基本的研究とバイオテクノロジーのためのNCAAの組み込み能力を大幅に向上させます.