マウス脳空間細胞タイプ分解能トランスクリプトーム発見を10×Visium空間トランスクリプトミクスおよびFLEX scRNA-seqを用いて評価するプロトコル

Understanding changes in gene expression and cell-cell signaling among spatial regions in diseased tissues adds critical biological information to understanding mechanisms. Here, we present a protocol to investigate molecular transcriptional drivers within intact murine tissue using 10× Genomics Visium spatial transcriptomics and 10× Genomics FLEX single-cell RNA sequencing (scRNA-seq) data. We describe steps for collecting mouse brain tissue from multiple ages, processing samples, and mounting the tissue. We then detail procedures for staining, FLEX tissue section collection, fixation, dissociation, and cell storage. For complete details on the use and execution of this protocol, please refer to Burns et al.¹.