GDNFの多成分受容体の特徴化
J J Treanor1, L Goodman, F de Sauvage
1Department of Neuroscience, Genentech, Inc., South San Francisco, California 94080, USA.
Nature
|July 4, 1996
まとめ
膠質細胞系由来神経栄養因子 (GDNF) は,その作用のために新しいタンパク質であるGDNFR-alphaを必要とします. このタンパク質はRet受容体と複合体を形成し,神経細胞の生存と発達に不可欠なシグナル伝達経路を開始します.
科学分野:
- 神経科学は神経科学である.
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- 膠質細胞系由来神経栄養因子 (GDNF) は,神経細胞の生存と発達に不可欠である.
- GDNFの作用の正確なメカニズムは,ほとんど不明のままでした.
- GDNFは,中枢神経系と外周神経系,腎臓と腸内神経系の発達において重要な役割を果たします.
研究 の 目的:
- グリアル細胞系由来神経栄養因子 (GDNF) 信号伝達の基礎にある分子メカニズムを解明する.
- GDNF媒介の生理学的反応に関与する受容体成分を特定する.
主な方法:
- グリアル細胞系由来神経栄養因子 (GDNF) の結合相互作用の特徴.
- GDNFシグナル伝達における新しいグリコシル・フォスファティディル・イノシトール (GPI) 関連タンパク質 (GDNFR-α) の役割の調査.
- GDNFR-αとRet受容体チロシンキナーゼの間の物理的な関連性の分析.
主要な成果:
- 新種のグリコシル・フォスファティディル・イノシトール (GPI) 関連タンパク質であるGDNFR-alphaは,高親和性Glial細胞系由来神経栄養因子 (GDNF) 結合タンパク質として特定されました.
- GDNFR-αは,GDNFに反応する細胞で発現する.
- 膠質細胞系由来神経栄養因子 (GDNF) は,GDNFR-alphaと孤児のチロシンキナーゼ受容体Retとの間の複合体の形成を誘導し,Retチロシンリン酸化につながります.
結論:
- 膠質細胞系派生神経栄養因子 (GDNF) は,そのシグナル伝達のために複数のサブユニットの受容体複合体を利用します.
- GDNFR-alphaはリガンド結合成分として機能し,Ret受容体は信号変換成分として機能する.
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