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rabindra.tharu.np@gmail.com
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Giant viruses as reservoirs of antibiotic resistance genes
Nucleocytoplasmic large DNA viruses (NCLDVs; also called giant viruses), constituting the phylum Nucleocytoviricota, can infect a wide range of eukaryotes and exchange genetic material with not only their hosts but also prokaryotes and phages. A few NCLDVs were reported to encode genes conferring resistance to beta‑lactam, trimethoprim, or pyrimethamine, suggesting that they are potential vehicles…
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rabindra.tharu.np@gmail.com
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Stabilized Cu(δ+)-OH species on in situ reconstructed Cu nanoparticles for CO(2)-to-C(2)H(4) conversion in neutral media
Achieving large-scale electrochemical CO reduction to multicarbon products with high selectivity using membrane electrode assembly (MEA) electrolyzers in neutral electrolyte is promising for carbon neutrality. However, the unsatisfactory multicarbon products selectivity and unclear reaction mechanisms in an MEA have hindered its further development. Here, we report a strategy that manipulates the interfacial microenvironment of Cu…
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rabindra.tharu.np@gmail.com
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An intronic copy number variation in Syntaxin 17 determines speed of greying and melanoma incidence in Grey horses
The Greying with age phenotype in horses involves loss of hair pigmentation whereas skin pigmentation is not reduced, and a predisposition to melanoma. The causal mutation was initially reported as a duplication of a 4.6 kb intronic sequence in Syntaxin 17. The speed of greying varies considerably among Grey horses. Here we demonstrate the presence of…
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rabindra.tharu.np@gmail.com
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Visualization of the Cdc48 AAA+ ATPase protein unfolding pathway
The Cdc48 AAA+ ATPase is an abundant and essential enzyme that unfolds substrates in multiple protein quality control pathways. The enzyme includes two conserved AAA+ ATPase motor domains, D1 and D2, that assemble as hexameric rings with D1 stacked above D2. Here, we report an ensemble of native structures of Cdc48 affinity purified from budding…
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rabindra.tharu.np@gmail.com
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Covariation of hot spring geochemistry with microbial genomic diversity, function, and evolution
The geosphere and the microbial biosphere have co-evolved for ~3.8 Ga, with many lines of evidence suggesting a hydrothermal habitat for life’s origin. However, the extent that contemporary thermophiles and their hydrothermal habitats reflect those that likely existed on early Earth remains unknown. To address this knowledge gap, 64 geochemical analytes were measured and 1022…
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rabindra.tharu.np@gmail.com
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Immunometabolic effects of lactate on humoral immunity in healthy individuals of different ages
Aging is characterized by chronic systemic inflammation and metabolic changes. We compare the metabolic status of B cells from young and elderly donors and found that aging is associated with higher oxygen consumption rates, and especially higher extracellular acidification rates, measures of oxidative phosphorylation and of anaerobic glycolysis, respectively. Importantly, this higher metabolic status, which…
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rabindra.tharu.np@gmail.com
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Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality
Enhancing thermogenic brown adipose tissue (BAT) function is a promising therapeutic strategy for metabolic disease. However, predominantly thermoneutral modern human living conditions deactivate BAT. We demonstrate that selective adipocyte deficiency of the oxygen-sensor HIF-prolyl hydroxylase (PHD2) gene overcomes BAT dormancy at thermoneutrality. Adipocyte-PHD2-deficient mice maintain higher energy expenditure having greater BAT thermogenic capacity. In human…
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rabindra.tharu.np@gmail.com
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Potassium dependent structural changes in the selectivity filter of HERG potassium channels
The fine tuning of biological electrical signaling is mediated by variations in the rates of opening and closing of gates that control ion flux through different ion channels. Human ether-a-go-go related gene (HERG) potassium channels have uniquely rapid inactivation kinetics which are critical to the role they play in regulating cardiac electrical activity. Here, we…
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rabindra.tharu.np@gmail.com
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Underlying dimensions of real-time word recognition in cochlear implant users
Word recognition is a gateway to language, linking sound to meaning. Prior work has characterized its cognitive mechanisms as a form of competition between similar-sounding words. However, it has not identified dimensions along which this competition varies across people. We sought to identify these dimensions in a population of cochlear implant users with heterogenous backgrounds…
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rabindra.tharu.np@gmail.com
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The temporal dynamics of group interactions in higher-order social networks
Representing social systems as networks, starting from the interactions between individuals, sheds light on the mechanisms governing their dynamics. However, networks encode only pairwise interactions, while most social interactions occur among groups of individuals, requiring higher-order network representations. Despite the recent interest in higher-order networks, little is known about the mechanisms that govern the formation…
