灵长类动物基因表达模式的内部和跨种类变异.
Wolfgang Enard1, Philipp Khaitovich, Joachim Klose
1Max-Planck-Institute for Evolutionary Anthropology, Inselstrasse 22, D-04103 Leipzig, Germany.
概括
人类和黑猩猩的基因表达显著不同,特别是在大脑中. 这项研究揭示了人类基因和蛋白质表达的明显变化,有助于物种差异.
科学领域:
- 进化生物学是进化的生物学.
- 基因组学就是基因组学.
- 进行比较的转录学.
背景情况:
- 人类和黑猩猩共享98.7%的基因组DNA.
- 尽管有很高的遗传相似性,但存在显著的形态,行为和认知差异.
- 改变基因表达是这些物种特异性特征的潜在驱动因素.
研究的目的:
- 研究人类,黑猩猩,猩猩和的特定物种基因和蛋白质表达模式.
- 为了确定人类与他们最近的亲属之间的差异的遗传基础.
- 检查基因表达的进化变化,特别是在人类大脑.
主要方法:
- 在血液白细胞,肝脏和大脑组织中使用微阵列进行比较转录组分析.
- 使用二维凝电泳对人类和黑猩猩进行比较蛋白质组分析.
- 包括三种小鼠物种来建模进化距离.
主要成果:
- 在研究的灵长类动物中确定了不同的,特定于物种的基因表达特征.
- 观察到人类大脑中基因和蛋白质表达的特别明显的变化.
- 即使在密切相关的物种中,也显示出基因表达模式的显著分歧.
结论:
- 基因和蛋白质表达的变化在人类进化中起着至关重要的作用.
- 人类大脑在基因表达水平上表现出独特和加速的进化变化.
- 比较转录组学和蛋白组学是理解物种分歧的强大工具.
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