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相关概念视频

What is Genetic Engineering?00:49

What is Genetic Engineering?

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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人工基因电路的工程设计

Jeff Hasty1, David McMillen, J J Collins

  • 1Department of Bioengineering, University of California San Diego, La Jolla, California 92093, USA. hasty@ucsd.edu

Nature
|November 15, 2002
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概括
此摘要是机器生成的。

后基因组研究重点是基因和蛋白质网络. 开发合成基因网络的数学框架使我们能够理解细胞动态,并创建新的生物控制系统.

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科学领域:

  • 系统生物学 系统生物学
  • 合成生物学 合成生物学
  • 生物工程是生物工程.

背景情况:

  • 后基因组研究旨在从基因和蛋白质相互作用中阐明细胞功能.
  • 分子网络是复杂的,需要数学框架来系统地理解.
  • 工程原理为通过子模块分析这些网络提供了一条途径.

研究的目的:

  • 建立一个数学框架,以了解细胞现象中的基因和蛋白质连接.
  • 利用最近在测序和基因工程方面的进展来创建合成基因网络.
  • 促进基因电路学科的出现,用于预测和评估细胞动态.

主要方法:

  • 设计和实施合成基因网络.
  • 将数学建模和定量分析应用于这些网络.
  • 从工程角度分析分子网络的底层子模块.

主要成果:

  • 证明了构建和分析合成基因网络的可行性.
  • 标志着基因电路学科的出现.
  • 为新的细胞控制逻辑形式铺平了道路.

结论:

  • 合成基因网络为了解细胞过程提供了一个强大的框架.
  • 这种方法促进了对生物系统的预测模型的开发.
  • 应用包括功能性基因组学,纳米技术以及基因和细胞疗法.