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相关概念视频

Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...

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相关实验视频

Updated: May 8, 2026

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model
08:46

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

Published on: September 29, 2011

在老化的酵母中增加基因组不稳定性.

Serge Gravel1, Stephen P Jackson

  • 1The Wellcome Trust/Cancer Research, UK.

Cell
|October 9, 2003
PubMed
概括

陈旧的酵母细胞显示,由于DNA双链断裂修复受损,异构性损失增加. 这项研究揭示了老化如何导致真核细胞的基因组不稳定.

科学领域:

  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 基因组不稳定是衰老的一个标志.
  • 了解与年龄相关的基因组不稳定性背后的分子机制至关重要.

研究的目的:

  • 研究酵母老化和基因组不稳定之间的关系.
  • 为了确定老细胞中受影响的特定DNA修复过程.

主要方法:

  • 使用了酵母模型生物 (Saccharomyces cerevisiae).
  • 评估了老年细胞与年轻细胞中异构性丧失的比率.
  • 检查了DNA双链断裂检测和修复途径.

主要成果:

  • 陈旧的酵母细胞表现出明显更高的异性损失率.
  • 这种增加与检测和修复DNA双链断裂的能力下降有关.
  • 有证据表明,随着细胞的衰老,DNA修复效率下降.

结论:

  • 酵母的衰老与基因组不稳定性增加有关.
  • 缺陷的DNA双链断裂修复是导致这种不稳定的关键因素.

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Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae
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Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae

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相关实验视频

Last Updated: May 8, 2026

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model
08:46

Studying Age-dependent Genomic Instability using the S. cerevisiae Chronological Lifespan Model

Published on: September 29, 2011

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
10:41

Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast

Published on: August 20, 2013

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae
11:08

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae

Published on: October 16, 2014

  • 这些发现提供了对衰老过程及其对真核细胞基因组完整性的影响的见解.