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相关概念视频

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Autoregulation of Blood Flow01:17

Autoregulation of Blood Flow

Autoregulation mechanisms are characterized by their inherent capacity for self-regulation without necessitating specific nervous stimulation or endocrine control. These mechanisms facilitate the adjustment of blood flow and, therefore, perfusion specific to each tissue region. This self-regulation encompasses chemical signals and myogenic controls.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation.

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相关实验视频

Updated: Jul 20, 2026

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

微RNAs调节了血液生成系的分化.

Chang-Zheng Chen1, Ling Li, Harvey F Lodish

  • 1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|December 6, 2003
PubMed
概括

微RNAs (miRNAs) 调节基因表达. 这项研究发现一种特定的miRNA,miR-181,促进了小鼠血液形成中的B淋巴细胞发育.

科学领域:

  • 分子生物学分子生物学
  • 发展生物学 发展生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 微RNAs (miRNAs) 是小的调节性RNA分子,参与基因表达.
  • 这些分子在各种生物体,包括植物和动物的发展中起着至关重要的作用.
  • 已知特定的miRNAs通过向信使RNA来通过转录后调节基因表达.

研究的目的:

  • 为了研究微RNAs在哺乳动物血液形成中的作用.
  • 为了确定血液细胞表达的特定miRNAs及其在发育过程中的动态调节.
  • 确定microRNAs对造血干细胞和祖细胞分化的功能影响.

主要方法:

  • 在造血细胞中的miRNAs的识别和表征.
  • 在血液形成和血统承诺期间分析miRNA表达模式.
  • 特定的miRNAs (例如,miR-181) 在造血干细胞/原始细胞中的异位表达.
  • 在体外和体内对B淋巴细胞分化的评估.

主要成果:

  • 三种miRNA被确定在血液细胞中具有特定的表达,在早期血液形成过程中被动态调节.
  • 发现miR-181在小鼠骨髓中的B淋巴细胞中优先表达.

更多相关视频

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein
09:48

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Published on: July 27, 2016

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

相关实验视频

Last Updated: Jul 20, 2026

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein
09:48

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Published on: July 27, 2016

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

  • 在分化试验和成年小鼠中,miR-181的子宫外表达增加了B系细胞的分数.

    • 结论:

    • 微RNA是控制小鼠血液形成的分子电路的组成部分.
    • miR-181在促进B淋巴细胞发育方面发挥着重要作用.
    • 这些发现表明,其他微RNA可能在脊椎动物发育中具有类似的调节功能.