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Updated: Jul 19, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

排序酶介导蛋白质结合:一种用于蛋白质工程的新方法.

Hongyuan Mao1, Scott A Hart, Amy Schink

  • 1Ansata Therapeutics, Inc. 505 Coast Boulevard South, La Jolla, California 92037, USA. Helen@ansatainc.com

Journal of the American Chemical Society
|March 5, 2004
PubMed
概括

排列酶 (SrtA) 酶通过创建氨基酸键,使体内有效的蛋白质结合成为可能. 这种强大的方法允许各种分子与蛋白质结合,从而推进生物结合技术.

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Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems07:35

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...