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相关概念视频

Nucleic acids02:43

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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相关实验视频

Updated: Jul 15, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

伪半结形成与RNA.

D J Ecker1, T A Vickers, T W Bruice

  • 1ISIS Pharmaceuticals, Carlsbad, CA 92008.

Science (New York, N.Y.)
|August 14, 1992
PubMed
概括

研究人员开发了一种伪半结图案,用于绑定RNA毛环,就像HIV TAR元素一样. 这种结合破坏了病毒转运激活蛋白相互作用,为向调节性RNA结构提供了新的策略.

科学领域:

  • 分子生物学分子生物学
  • 病毒学 病毒学
  • 生物化学 生物化学

背景情况:

  • 人类免疫缺陷病毒 (HIV) TAR元素是病毒复制必不可少的关键RNA结构.
  • 该 TAR 元素作为病毒转活蛋白 (Tat) 的结合部位.
  • 向RNA结构是病毒感染的潜在治疗策略.

研究的目的:

  • 为了研究伪半结结合图案的实用性,以准RNA毛环.
  • 使用这个动机来评估HIV TAR元素结构和Tat结合的破坏.
  • 探索这种基因在抑制病毒转活化的潜力.

主要方法:

  • 设计和合成针对HIV TAR元素的寡核酸.
  • 寡核酸的不对称结合形成了一个伪半结结构.
  • 对对称与不对称的结合性寡核酸的亲和度测量.
  • 评估Tat衍生的与修改的TAR结构的结合.

主要成果:

  • 不对称结合的寡核酸形成伪半结,对 TAR 元素有较高的亲和力.
  • 伪半结图案显著改变了TAR结构.
  • 伪半结的寡核酸抑制了Tat衍生的酸结合,破坏了TAR结构.

更多相关视频

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

相关实验视频

Last Updated: Jul 15, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

结论:

  • 伪半结是准和破坏RNA毛结构的有效动机.
  • 这一策略可以破坏重要的病毒RNA-蛋白相互作用,例如Tat与HIV TAR元素的结合.
  • 伪半结图案有望通过准调节性RNA结构来开发针对病毒的新疗法.