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相关概念视频

Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

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相关实验视频

Updated: Jun 25, 2026

Pull-down of Calmodulin-binding Proteins
07:51

Pull-down of Calmodulin-binding Proteins

Published on: January 23, 2012

通过单分子光谱学采样calmodulin中的展开中间体.

Brian D Slaughter1, Jay R Unruh, E Shane Price

  • 1Department of Chemistry, 1251 Wescoe Hall Drive, University of Kansas, Lawrence, Kansas 66045-7582, USA.

Journal of the American Chemical Society
|August 25, 2005
PubMed
概括

单分子FRET揭示了calmodulin (CaM) 通过中间状态展开,而不是简单的两种状态过程. 和2+) 结合的CaM在用尿素去化过程中表现出明显的中间形状.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 生物物理学的生物物理.

背景情况:

  • 卡尔莫杜林 (CaM) 是一种重要的信号蛋白.
  • 了解CaM的结构动态对于其功能至关重要.
  • 变性研究揭示了蛋白质折叠/展开的途径.

研究的目的:

  • 描述CaM的脱和部分脱状态.
  • 为了研究Apo和Ca2+) 结合的CaM的展开途径.
  • 在CaM变性化过程中识别中间形态状态.

主要方法:

  • 单对光共振能量转移 (spFRET) 的测量.
  • 使用一个双重标记的CaM突变 (CaM-T34C-T110C).
  • 使用不同度的去色剂尿素.

主要成果:

  • 根据尿素度观察到多个CaM构造状态.
  • CaM 化不遵循两种状态模型,表示中间状态.
  • 对于apoCaM和Ca(2+) -CaM,已经确定了明显的中间形状.

结论:

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Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
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Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and &lambda;) Hyperspectral FRET Imaging and Analysis
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Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis

Published on: October 27, 2020

相关实验视频

Last Updated: Jun 25, 2026

Pull-down of Calmodulin-binding Proteins
07:51

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Published on: January 23, 2012

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
08:22

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Published on: July 17, 2018

Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and &lambda;) Hyperspectral FRET Imaging and Analysis
08:22

Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis

Published on: October 27, 2020

  • CaM通过至少一个中间状态展开.
  • 为 CaM 提出了一种逐步,顺序的域展开模型.
  • 不同的中间体的存在凸显了依赖Ca2+的结构变化.