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相关概念视频

Mismatch Repair01:36

Mismatch Repair

43.7K
Overview
43.7K
Mismatch Repair01:20

Mismatch Repair

6.6K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
6.6K
Overview of DNA Repair02:25

Overview of DNA Repair

33.8K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
33.8K
Base Excision Repair01:54

Base Excision Repair

26.4K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
26.4K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

40.9K
Overview
40.9K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

5.2K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.2K

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Comparative Analysis of Ultra-Low Versus Hydrogel Growing Conditions for Patient-Derived Glioma Organoids.

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相关实验视频

Updated: Feb 8, 2026

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

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在人类不匹配修复中,MutLalpha的内核分解功能.

Farid A Kadyrov1, Leonid Dzantiev, Nicoleta Constantin

  • 1Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.

Cell
|July 29, 2006
PubMed
概括

人类MutLalpha (MLH1*PMS2) 在DNA不匹配修复中充当潜伏内核酶. 它与其他蛋白质一起变得活跃,以启动DNA切除,这对于遗传性非多重性结肠癌至关重要.

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 癌症研究 癌症研究

背景情况:

  • 遗传性非多重性结肠癌 (HNPCC) 与MutLalpha (MLH1*PMS2) 突变有关.
  • 穆特拉尔法在DNA不匹配修复中的确切作用仍然不完全理解.

研究的目的:

  • 阐明人类MutLalpha在DNA不匹配修复中的功能.
  • 确定MutLalpha在不匹配修复过程中启动DNA切除的机制.

主要方法:

  • 采用纯化蛋白质 (MutLalpha,MutSalpha,RFC,PCNA) 和DNA基质进行体外生化分析.
  • 位点定向突变发生,以调查内核酶活性位点.
  • 对PMS2同类和细菌MutL蛋白的比较分析.

主要成果:

  • 人类MutLalpha作为潜伏的内核酶起作用,由一个不匹配的复合体,MutSalpha,RFC,PCNA和ATP激活.
  • 含有MutLalpha的系统在不匹配附近的切断链上切割DNA异质复合体.
  • 被MutSalpha激活的外核酶I,可以去除含有不匹配的DNA段.
  • 一个保存的PMS2基因 (DQHA(X) ((2) E) ((X) ((4) E) 可能是内核酶活性位点.

更多相关视频

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

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Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis
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Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis

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相关实验视频

Last Updated: Feb 8, 2026

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

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Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis
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Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis

Published on: February 9, 2010

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结论:

  • 穆特拉尔法的内核酶活性对于在不匹配修复过程中启动DNA切割至关重要.
  • 鉴定到的活性部位动机表明了进化保护和物种间不匹配修复机制的潜在差异.
  • 了解MutLalpha的功能为我们提供了有关HNPCC病变的见解.