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相关概念视频

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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相关实验视频

Updated: May 10, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

人类CtIP促进了DNA末端切除.

Alessandro A Sartori1, Claudia Lukas, Julia Coates

  • 1The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.

Nature
|October 30, 2007
PubMed
概括

人类CtIP蛋白对于DNA双链断裂 (DSB) 修复至关重要,通过促进DSB切除和同源重组. 这种蛋白质对于细胞循环检查点信号至关重要,其功能在物种之间得到保护.

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Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

相关实验视频

Last Updated: May 10, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
14:49

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
11:11

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 遗传学 是一个遗传学.

背景情况:

  • DNA双链断裂 (DSB) 触发细胞周期检查点信号和修复机制.
  • 众所周知,MRE11复合体参与了DSB处理.

研究的目的:

  • 研究人类CTIP (RBBP8) 在DNA双链断裂 (DSB) 修复中的作用.
  • 阐明CtIP,MRE11复合体和同源重组之间的关系.

主要方法:

  • 对CtIP招募到DSB的细胞周期分析.
  • 评估CtIP在DSB切除和RPA/ATR招募中的作用.
  • 同免疫沉试验用于研究CtIP-MRE11相互作用.
  • 同类重组试验. 同类重组试验.
  • 用酵母Sae2进行序列同质分析.

主要成果:

  • CtIP赋予了对DSB诱导剂的耐药性,并在S和G2阶段被DSB招募.
  • CtIP对于DSB切除至关重要,导致RPA和ATR招募和ATR激活.
  • 在物理和功能上,CTIP与MRE11复合体相互作用.
  • 对于高效的同源重组,CtIP和MRE11都需要.
  • CtIP显示出与酵母Sae2的序列同质性,表明功能保留.

结论:

  • 在DSB切除,检查点信号和同源重组中,CTIP起着至关重要的作用.
  • CtIP与MRE11复合体协同工作,以实现高效的DSB维修.
  • 类似CTIP的蛋白质在DNA修复途径中具有进化保存的作用.