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相关概念视频

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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相关实验视频

Updated: Jun 22, 2026

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

对于微RNA来说,这是一个死胡同.

René F Ketting1

  • 1Hubrecht Institute, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands. ketting@niob.knaw.nl

Cell
|December 28, 2007
PubMed
概括
此摘要是机器生成的。

死亡端 (Dnd1) 蛋白质对于生殖细胞的生存和预防瘤至关重要. 新的研究表明,Dnd1通过阻断传递 RNA 的微RNA 沉默来起作用,确保基因表达.

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mirMachine: A One-Stop Shop for Plant miRNA Annotation
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mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

相关实验视频

Last Updated: Jun 22, 2026

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

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科学领域:

  • 发育生物学是发展生物学.
  • 分子生物学分子生物学
  • 癌症研究 癌症研究

背景情况:

  • 死亡点 (Dnd1) 是一种必不可少的RNA结合蛋白.
  • Dnd1在维持生殖细胞活力方面发挥着至关重要的作用.
  • 已知Dnd1可以抑制生殖细胞瘤的形成.

研究的目的:

  • 阐明Dnd1发挥其功能的分子机制.
  • 为了研究Dnd1和微RNA介导的基因沉默之间的关系.
  • 了解Dnd1如何抵消生殖细胞中的microRNA活性.

主要方法:

  • 这项研究可能涉及分子生物学技术,以评估RNA-蛋白相互作用.
  • 实验可能集中在分析mRNA和microRNA表达水平上.
  • 研究Dnd1对微RNA点的影响可能是关键方法.

主要成果:

  • 有证据表明Dnd1直接干扰微RNA介导的mRNAs沉默.
  • Dnd1似乎可以保护特定的mRNA免受微RNA的降解或转化抑制.
  • 这种对抗机制被提议为Dnd1在生殖细胞活力和瘤抑制中的作用提供依据.

结论:

  • Dnd1在生殖细胞中的功能至少部分通过对抗microRNA沉默来调解.
  • 这种机制解释了Dnd1如何支持胚胎细胞的发育,并防止瘤发生.
  • 这些发现提供了对生殖线发育中的转录后基因调节的新见解.