MONOPTEROS通过调节移动转录因子来控制胚胎根的启动.
Alexandra Schlereth1, Barbara Möller, Weilin Liu
1Entwicklungsgenetik, Zentrum für Molekularbiologie der Pflanzen (ZMBP), Universität Tübingen, Auf der Morgenstelle 3, 72076 Tübingen, Germany.
Nature
|March 12, 2010
概括
植物根的发育取决于位置信号. 研究人员确定了TARGET OF MP (TMO) 5和TMO7作为新的细胞间信号,对于指定Arabidopsis胚胎根创始细胞至关重要.
科学领域:
- 植物发育生物学植物发育生物学
- 分子遗传学 分子遗传学
- 植物信号通道的信号通道.
背景情况:
- 植物细胞认同依赖于定位信息和细胞间通信来形成发育模式.
- 在阿拉比多普西斯胚胎发生过程中,由邻近胚胎细胞发出的信号来指定下细胞 (初级根介质系统创始细胞).
- 已知依赖auxin的转录因子MONOPTEROS (MP) 通过auxin传输驱动低分离特异,但需要额外的信号.
研究的目的:
- 为了确定从胚胎到低分泌前体的细胞间信号传递的新型MP依赖基因.
- 为了阐明这些基因在阿拉比多普西斯胚胎根特征中的作用.
主要方法:
- 基于微阵列的基因表达分析,以隔离MP目标基因.
- 在Arabidopsis突变体中识别的目标基因的遗传分析.
- 对TMO7.7的蛋白质定位和运动的分析.
主要成果:
- 确定了TARGET OF MP 5 (TMO5) 和TARGET OF MP 7 (TMO7) 作为MP的直接转录目标.
- TMO5和TMO7对bHLH转录因子进行编码,这些转录因子表达在与低细胞相邻的胚胎细胞中.
- 对于MP依赖的根启动,TMO5和TMO7是必需的,并且部分充足.
- TMO7作为一个移动的细胞间信号,从胚胎移动到催眠前体.
结论:
- TMO5和TMO7是MP依赖信号通路的关键组成部分,用于Arabidopsis的胚胎根特征.
- TMO7代表了一种新型的MP-依赖细胞间信号分子,参与植物发育.
- 了解这种信号通路,可以了解植物胚胎发生过程中细胞命运决定的机制.
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