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相关概念视频

Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
The Proteasome Structure01:17

The Proteasome Structure

The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...

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In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
11:36

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Published on: July 25, 2019

乌比奎丁:相同的分子,不同的降解途径.

Michael J Clague1, Sylvie Urbé

  • 1Institute of Translational Medicine, University of Liverpool, UK. clague@liv.ac.uk

Cell
|November 30, 2010
PubMed
概括
此摘要是机器生成的。

乌比基标记针对蛋白质通过蛋白质体,溶解体或自体进行降解. 链条的长度和链接决定了特定的降解途径,影响相互作用和二维基因酶活性.

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Ubiquitin Chain Analysis by Parallel Reaction Monitoring
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Last Updated: Jun 6, 2026

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
11:36

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Ubiquitin Chain Analysis by Parallel Reaction Monitoring
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科学领域:

  • 细胞生物学 细胞生物学
  • 生物化学 生物化学
  • 分子生物学分子生物学

背景情况:

  • 乌比奎丁 (Ub) 是一个关键的翻译后修饰.
  • 乌比奎丁结合向基质进行降解.
  • 哺乳动物细胞利用三个主要的蛋白质降解途径:蛋白质体,溶解体和自体.

研究的目的:

  • 调查乌比奎在基质向不同降解途径中的作用.
  • 探索如何影响全方位链特征的途径选择.

主要方法:

  • 对基链长度和链接类型的分析.
  • 评估基质与特定路径受体的相互作用.
  • 评估对二维基酶 (DUB) 酶的敏感性.

主要成果:

  • 乌比基作为所有三个主要降解路径的共同信号.
  • 乌比奎丁链的特性,包括长度和链接,决定了基质的命运.
  • 这些特性调节与特定受体和DUBs的相互作用.

结论:

  • 乌比基链特征是蛋白质降解路径选择的关键决定因素.
  • 不同的无处不在模式确保了蛋白酶体,溶酶体和自酶体的基质特异性.
  • 了解这些机制对于理解细胞蛋白质平衡至关重要.