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相关概念视频

Internal Receptors01:31

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
Short-distance Transport of Resources02:12

Short-distance Transport of Resources

Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Channel Rhodopsins01:11

Channel Rhodopsins

Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...

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相关实验视频

Updated: May 8, 2026

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
09:27

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications

Published on: May 10, 2016

通过BRI1对Brassinosteroid感知的结构洞察力.

Ji She1, Zhifu Han, Tae-Wuk Kim

  • 1Key Laboratory for Protein Sciences of Ministry of Education School of Life Sciences, Tsinghua University, Beijing 100084, China.

Nature
|June 14, 2011
PubMed
概括
此摘要是机器生成的。

植物激素称为素类固醇是生长的关键. 研究人员通过结构分析发现了BRI1受体是如何通过结构分析识别布拉西诺利德的,揭示了植物激素信号传递的分子基础.

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A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana
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BRET-based G Protein Biosensors for Measuring G Protein-Coupled Receptor Activity in Live Cells
09:21

BRET-based G Protein Biosensors for Measuring G Protein-Coupled Receptor Activity in Live Cells

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相关实验视频

Last Updated: May 8, 2026

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
09:27

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications

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A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana
07:07

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科学领域:

  • 植物生物学 植物生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 铜类固醇是重要的植物激素,调节生长和发育.
  • 激素的感知涉及到BRASSINOSTEROID-INSENSITIVE 1 (BRI1) 受体复合体. 这种复合体可以产生激素.
  • BRI1通过其细胞外氨酸丰富的重复 (LRR) 域识别氨类固醇,启动信号级联.

研究的目的:

  • 为了阐明由BRI1.1.识别布拉西诺固醇的分子机制.
  • 确定 BRI1 LRR 域在自由状态和联结状态中的晶体结构.
  • 为了提供关于类固醇诱导的受体激活的结构性见解.

主要方法:

  • 使用X射线晶体学来确定BRI1的结构.
  • 结构分析 BRI1 ((LRR) 在阿波和布拉西诺利德结合形式.
  • 生物化学测试以确认单体状态和连接物结合.

主要成果:

  • BRI1 ((LRR) 作为一个单体,独立于黄铜化物结合.
  • 结构揭示了一个螺旋状电磁带,有一个插入域,形成一个结合槽.
  • 布拉西诺利德通过诱导适合机制在这个槽内结合,稳定了域间循环.

结论:

  • 这项研究定义了由BRI1受体识别布拉西诺固醇的结构基础.
  • 诱导适应机制涉及循环稳定,促进激素结合.
  • 这些发现提供了关键的洞察力brassinosteroid信号和受体激活的初始步骤.