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相关概念视频

General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

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相关实验视频

Updated: May 17, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Na&#239;ve CD4+ T Cells Using a TGF-&#946;-containing Protocol
08:20

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

新的Foxo1-依赖的转录程序控制T(reg) 细胞功能.

Weiming Ouyang1, Will Liao, Chong T Luo

  • 1Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, USA.

Nature
|November 9, 2012
PubMed
概括
此摘要是机器生成的。

叉头盒O1 (Foxo1) 对于调节T (Treg) 细胞功能至关重要,维持免疫恒常. 在Treg细胞中删除Foxo1会导致严重的炎症,突出显示了它在Treg细胞最初结合之外的重要作用.

更多相关视频

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
15:33

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

相关实验视频

Last Updated: May 17, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Na&#239;ve CD4+ T Cells Using a TGF-&#946;-containing Protocol
08:20

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
15:33

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 通过Foxp3标记的调节性T (Treg) 细胞通过抑制免疫反应来维持免疫平衡.
  • 虽然Akt酶和Foxo转录因子调节Treg细胞的早期结合,但它们在成熟的Treg细胞功能中的作用尚不清楚.

研究的目的:

  • 调查FOXO蛋白在Treg细胞平衡中的作用以及超越承诺阶段的功能.
  • 为了确定Foxo1是否调节Treg细胞功能和免疫平衡.

主要方法:

  • 产生了具有Treg细胞特异缺失Foxo1.1的小鼠.
  • 分析了Treg细胞功能,免疫平衡和炎症反应.
  • 对Foxo1结合部位进行了全基因组分析.

主要成果:

  • 特雷格细胞表现出高的Foxo1水平和减少的Akt激活,Foxo1酸化和核排斥.
  • 对Treg细胞特异的Foxo1缺失导致致命的炎症性疾病,类似于Foxp3缺乏,但没有Treg细胞损失.
  • 鉴定了约300个Foxo1点基因,包括Ifng,Foxp3.3没有直接调节.

结论:

  • 福克索1是Treg细胞功能和免疫平衡的关键调节者.
  • 这种Akt-Foxo1信号通路控制了一种对Treg细胞功能至关重要的新型遗传程序.
  • 福克索1在维持免疫平衡方面发挥着至关重要的作用,独立于福克索3在Treg细胞数量中的作用.