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相关概念视频

Role of ER in the Secretory Pathway01:17

Role of ER in the Secretory Pathway

Eukaryotic cells have a special pathway that enables communication between various intracellular membrane-bound compartments and also with the extracellular environment. This pathway is termed as the secretory pathway.
Components of the secretory pathway
About a third of proteins synthesized in the cell are sorted via the secretory route. They shuffle between different compartments in membrane-bound vesicles until they reach their final destination. The main intracellular compartments involved...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
The Endoplasmic Reticulum01:43

The Endoplasmic Reticulum

The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
ER Retrieval Pathway01:45

ER Retrieval Pathway

In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...

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相关实验视频

Updated: May 11, 2026

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

微RNAs访问了急救中心.

Taiowa A Montgomery1, Gary Ruvkun

  • 1Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114, USA.

Cell
|April 30, 2013
PubMed
概括
此摘要是机器生成的。

现在已知植物微RNAs (miRNAs) 能够调节内质网膜内的基因标. 这项研究确定了植物miRNA途径中涉及基因沉默的新组成部分.

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Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
13:36

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

相关实验视频

Last Updated: May 11, 2026

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation
13:36

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

科学领域:

  • 分子生物学分子生物学
  • 植物科学 植物科学
  • 遗传学 是一个遗传学.

背景情况:

  • 微RNAs (miRNAs) 是真核生物中基因表达的关键调节者.
  • 在许多途径中,miRNA介导的基因沉默的精确亚细胞定位仍然在很大程度上是未知的.
  • 了解miRNA标调节是解读基因表达控制的关键.

研究的目的:

  • 为了研究微RNA (miRNA) 发挥其调节功能的细胞位置.
  • 为了确定参与植物miRNA路径的新型组件.
  • 阐明特定有机体在miRNA介导的基因沉默中的作用.

主要方法:

  • 利用先进的分子生物学技术研究植物中的miRNA活性.
  • 研究了miRNA路径组件与细胞器官的相互作用.
  • 采用基因沉默测试来确认功能角色.

主要成果:

  • 确定了一种参与植物miRNA通路的新型蛋白质.
  • 证明了内质网膜是miRNA介导基因沉默的地点.
  • 提供了证据,证明了内分泌网膜在这个过程中的功能意义.

结论:

  • 细胞内膜网膜在植物miRNA介导的基因沉默中发挥着重要作用.
  • 这一发现扩大了我们对miRNA活动亚细胞局部化的理解.
  • 开辟了植物转录后基因调节研究的新途径.