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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.6K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.6K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.1K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.1K

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相关实验视频

Updated: Apr 14, 2026

Single Particle Cryo-Electron Microscopy: From Sample to Structure
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一个单粒子冷电子显微镜的入门书.

Yifan Cheng1, Nikolaus Grigorieff2, Pawel A Penczek3

  • 1Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA.

Cell
|April 25, 2015
PubMed
概括

单粒子冷电子显微镜 (cryo-EM) 确定没有晶体的蛋白质结构. 进步使近原子分辨率成为可能,但这种强大的技术仍然面临挑战.

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A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
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A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

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相关实验视频

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11:52

Single Particle Cryo-Electron Microscopy: From Sample to Structure

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A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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科学领域:

  • 结构生物学 结构生物学
  • 生物物理学的生物物理.
  • 生物化学 生物化学

背景情况:

  • 单粒子冷电子显微镜 (cryo-EM) 是一种强大的技术,用于确定蛋白质和宏分子复合体的三维结构.
  • 与X射线结晶学不同,冷EM不需要样品结晶.
  • 最近的探测器和软件技术进步显著提高了冷EM中的图像质量和分辨率.

研究的目的:

  • 为不熟悉该技术的科学家提供单粒子冷电磁场的概述.
  • 解释使用冷EM确定蛋白质结构所涉及的步骤和考虑因素.
  • 作为一个入门指南,用于新练习的冷EM.

主要方法:

  • 使用冷电子显微镜进行单粒子分析.
  • 数据处理涉及图像记录和计算算法.
  • 在接近原子分辨率的结构确定.

主要成果:

  • 可以获得生物大分子的高质量图像.
  • 接近原子分辨率的结构可以使用冷EM确定.
  • 该技术为结构确定提供了X射线结晶学的替代方案.

结论:

  • 低温EM是一种快速发展的结构生物学技术.
  • 了解这些步骤和挑战对于成功的结构确定至关重要.
  • 这本入门书为解释冷EM数据和练习该技术提供了一份指南.