通过Ca(2+) 的信号整合调节肠道干细胞活动.
Hansong Deng1, Akos A Gerencser1, Heinrich Jasper1
1The Buck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, California 94945, USA.
Nature
|December 4, 2015
概括
食L-谷氨酸增加了肠干细胞 (ISC) 分裂和Drosophila的肠道生长. (Ca2+) 信号传递,由metabotropic谷氨酸受体 (mGluRs) 调节,通过calcineurin和Crtc控制ISC的增殖.
科学领域:
- 干细胞生物学 干细胞生物学
- 信号传递的.
- 德洛索菲拉 (Drosophila melanogaster) 是一个有机体模型.
背景情况:
- 身体干细胞通过动态增殖和分化来维持组织平衡.
- 压力和代谢线索影响干细胞的行为.
- 肠道干细胞 (ISC) 对肠道健康和再生至关重要.
研究的目的:
- 为了确定肠道干细胞 (ISC) 活动的关键调节者在Drosophila.
- 阐明 (Ca2+) 信号在ISC扩散中的作用.
- 了解饮食线索如何调节ISC行为.
主要方法:
- 研究了Drosophila melanogaster作为一个模型系统.
- 使用食L-谷氨酸作为一种刺激剂.
- 检查了在ISC中metabotropic谷氨酸受体 (mGluRs) 的作用.
- 测量了细胞溶液Ca2+的振荡和度.
- 分析了calcineurin和CREB调节的转录协活性剂 (Crtc) 的参与.
主要成果:
- 饮食中的L-谷氨酸刺激ISC分裂和肠道生长.
- 在ISC中,转基因氨酸受体 (mGluRs) 对于这种反应至关重要.
- mGluR的激活调节细胞质Ca2+振荡,导致持续高的Ca2+水平.
- 高细胞质Ca2+诱导ISC通过calcineurin和Crtc.通过ISC扩散.
- ISC在Ca2+振荡状态之间呈现可逆转变,反映了扩散模式.
结论:
- (Ca2+) 信号传递是Drosophila.的肠干细胞 (ISC) 活动的中央调节者.
- 细胞内Ca2+的动态调节使ISC能够整合各种信号并适应增殖.
- 这种机制使ISC能够根据组织需求调整其增殖活动,以应对饮食和压力刺激.
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