Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Eukaryotic Compartmentalization01:37

Eukaryotic Compartmentalization

19.5K
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal...
19.5K
Eukaryotic Compartmentalizations01:46

Eukaryotic Compartmentalizations

182.1K
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
182.1K
Eukaryotic Compartmentalization01:46

Eukaryotic Compartmentalization

28.3K
28.3K
Subcellular Fractionation01:32

Subcellular Fractionation

9.5K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
9.5K
Fluid Mosaic Model01:19

Fluid Mosaic Model

19.2K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
19.2K
Distribution of Cytoplasmic Content02:33

Distribution of Cytoplasmic Content

4.8K
Cytokinesis segregates a cell’s chromosomes and organelles into its daughter cells. Organelles divide and grow prior to cell division but cannot be synthesized de novo; therefore, cells must receive at least one copy of each organelle to survive. Currently, many of the details of how the organelles are distributed are not yet fully elucidated.
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of...
4.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Large RNA polymerase II condensates are promoter-centric assemblies associated with early stages of transcription at all expressed genes.

bioRxiv : the preprint server for biology·2026
Same author

Active RNA synthesis patterns nuclear condensates.

Cell systems·2026
Same author

Spindle pole proteins confine chromosomes to ensure their expulsion during female meiosis.

bioRxiv : the preprint server for biology·2026
Same author

Nuclear tau aggregates inhibit RNA export and form by secondary seeding from cytosolic tau aggregates.

bioRxiv : the preprint server for biology·2026
Same author

Base Composition Influences the Position and Precision of RNA Polymerase II Disassociation in Basal and Perturbed Conditions.

bioRxiv : the preprint server for biology·2026
Same author

Polyserine domains are toxic and exacerbate tau pathology in mice.

Proceedings of the National Academy of Sciences of the United States of America·2026

相关实验视频

Updated: Mar 18, 2026

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.7K

阶段分离细胞体的组成控制

Salman F Banani1, Allyson M Rice1, William B Peeples1

  • 1Department of Biophysics and Howard Hughes Medical Institute, UT Southwestern Medical Center, Dallas, TX 75390, USA.

Cell
|July 5, 2016
PubMed
概括

科学家使用工程蛋白和RNA建模了P体和PML核体等细胞体. 他们发现可以通过改变架度来控制身体组成,

更多相关视频

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

5.6K
Cell Co-culture Patterning Using Aqueous Two-phase Systems
10:11

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

19.6K

相关实验视频

Last Updated: Mar 18, 2026

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.7K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

5.6K
Cell Co-culture Patterning Using Aqueous Two-phase Systems
10:11

Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

19.6K

科学领域:

  • 生物化学
  • 细胞生物学
  • 生物物理

背景情况:

  • 细胞体如P体和PML核体 (PML NBs) 是通过多价值相互作用组织的类似液体的结构.
  • 这些细胞体组成的一般原理尚不清楚.
  • 了解这些原理对于理解细胞的组织和功能至关重要.

研究的目的:

  • 研究分相细胞体的组成的一般原理.
  • 模拟和理解细胞体构成是如何由支架和客户端分子控制的.
  • 探索快速和可调节的细胞体组成的潜力.

主要方法:

  • 设计多价值蛋白和RNA分子以在体外和细胞内创建模型细胞体.
  • 研究了这些模型体内的相分离行为和客户端蛋白分离.
  • 分析了支架度和价值对人体构成的影响.
  • 将模型体与自然PMLNB和P体的分区行为进行比较.

主要成果:

  • 工程支架分子形成相隔液体体,集中低价值客户端蛋白质.
  • 客户端蛋白分区在相位图的对角线上呈现出明显的切换,这取决于支架比率.
  • 通过改变支架度或价值,可以快速切换细胞体组成.
  • 自然的PML NBs和P体表现出类似的分区行为,与PML SUMOylation和mRNA水平相关.

结论:

  • 提出了一个概念框架来理解由异型多价相互作用形成的细胞体的组成和控制.
  • 这些发现表明细胞体的组成是由支架分子的比率和度动态调节的.
  • 这项研究提供了PML NB和P体等自然细胞体如何维持和控制它们的组成的见解.