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相关概念视频

The DNA Replication Fork01:02

The DNA Replication Fork

41.4K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
41.4K
The DNA Replication Fork01:02

The DNA Replication Fork

18.8K
18.8K
Replication in Prokaryotes01:32

Replication in Prokaryotes

28.4K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
28.4K
Replication in Prokaryotes02:35

Replication in Prokaryotes

99.9K
Overview
99.9K
Replication in Eukaryotes02:31

Replication in Eukaryotes

206.2K
Overview
206.2K
Replication in Eukaryotes01:29

Replication in Eukaryotes

18.0K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
18.0K

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相关实验视频

Updated: Feb 24, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
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Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

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转录复制冲突:方向问题

Yea-Lih Lin1, Philippe Pasero1

  • 1Institute of Human Genetics, CNRS UMR9002 and University of Montpellier, Equipe Labéllisée Ligue Contre le Cancer, 34090 Montpellier, France.

Cell
|August 13, 2017
PubMed
概括

基因组不稳定源于DNA复制和转录之间的冲突. 研究表明,正面碰撞阻碍了DNA复制分叉的进展,而不是共同的碰撞.

科学领域:

  • 分子生物学
  • 遗传学
  • 基因组不稳定性

背景情况:

  • DNA复制和转录是细胞的基本过程.
  • 这些过程之间的干扰可能导致基因组不稳定.
  • 了解这种干扰的机制对于理解基因组维护至关重要.

研究的目的:

  • 研究复制-转录碰撞对DNA复制分叉进展的影响.
  • 为了区分正面碰撞和共同方向碰撞的影响.
  • 确定复制转录干扰的分子机制.

主要方法:

  • 对细菌和人类细胞中的DNA复制叉动态进行比较分析.
  • 研究RNA-DNA混合体 (R-循环) 在阻碍复制中的作用.
  • 使用遗传和分子生物学技术研究碰撞结果.

主要成果:

  • DNA复制和转录分叉之间的正面碰撞显著阻碍了分叉的进展.
  • 同方向的碰撞似乎不会在相同的程度上阻碍分叉的进展.
  • 形成RNA-DNA杂交 (R-循环) 是导致复制分叉停滞的关键机制.

结论:

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

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  • 面对面的复制-转录碰撞是基因组不稳定的重要来源.
  • 在正面碰撞时,R环形成是复制叉阻碍的关键媒介.
  • 这些发现为基因组维护和遗传不稳定的起源提供了洞察力.