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相关概念视频

Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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The Eukaryotic Promoter Region02:40

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The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
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The Eukaryotic Promoter Region02:40

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Co-activators and Co-repressors02:04

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Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
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相关实验视频

Updated: Feb 23, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
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聚类蛋白将PRC2复合体与CpG群岛连接起来

Haojie Li1, Robert Liefke2,3, Junyi Jiang1

  • 1Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, 19 Xinjiekouwai Avenue, Beijing 100875, China.

Nature
|September 5, 2017
PubMed
概括

聚合物样蛋白 (PCL) 对于将聚合物抑制复合物2 (PRC2) 引入CpG群岛至关重要. 这种结合机制涉及翼状螺旋结构,对于转录调节和维持细胞身份至关重要.

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科学领域:

  • 表观遗传学和转录调节
  • 染色体生物学
  • 基因沉默的分子机制

背景情况:

  • 多抑制复合体2 (PRC2) 对于基因沉默,细胞身份和分化至关重要.
  • 聚类 (PCL) 蛋白 (例如PHF1,MTF2,PHF19) 与PRC2结合,可能调节其活性或基因组向.
  • 在富含CpG的区域中,包括低DNA甲基化的CpG岛屿中,PRC2结合点被丰富,但招募机制尚不清楚.

研究的目的:

  • 阐明聚合物样蛋白 (PCL) 与富含CpG的DNA结合的结构基础和机制.
  • 确定PCL蛋白在聚合物抑制复合物2 (PRC2) 到CpG群岛的招募中的作用.
  • 了解PCL-DNA相互作用对体内转录调节的贡献.

主要方法:

  • 确定PHF1和MTF2与CpGDNA和H3K36me3结合的N端域的晶体结构.
  • 生物化学测试以描述DNA结合的特异性.
  • 在小鼠胚胎干细胞中对CpG岛屿促进体的PRC2招募的评估.

主要成果:

  • PHF1和MTF2的N端域采用了翼状螺旋结构,专门识别非甲基化CpG图案.
  • 这种DNA结合机制与传统的翼螺旋形状有很大不同.
  • 在小鼠胚胎干细胞中,PCL蛋白对PRC2向CpG岛屿促进体的有效招募至关重要.

结论:

  • PCL蛋白通过一种新的翼状螺旋结构直接结合非甲基化CpG基因.
  • PCL 蛋白质在将PRC2 向CpG岛屿方面发挥着至关重要的作用,这是一个关键的表观遗传调节机制.
  • 这项研究提供了PCL蛋白在PRC2招募和转录调节中的直接证据.