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相关概念视频

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

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Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent...
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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

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This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator...
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A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

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This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this...
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Generation of Genetically Modified Mice through the Microinjection of Oocytes10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

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The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping...
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DNA Vector-based RNA Interference to Study Gene Function in Cancer13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

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RNA interference (RNAi) possesses many advantages over gene knockout and has been broadly used as a tool in gene functional studies. The invention of DNA vector-based RNAi technology has made long term and inducible gene knockdown possible, and also increased the feasibility of gene silencing in...
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Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans10:58

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

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We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C.
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相关实验视频

Updated: Jan 15, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
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克隆非人类灵长类动物的定制卵细胞

Jose B Cibelli1, John B Gurdon2

  • 1Departments of Animal Science and Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA.

Cell
|February 10, 2018
PubMed
概括

研究人员成功地用胎儿纤维细胞克隆了两只子. 这一突破体细胞核转移 (SCNT) 是通过改善卵细胞的表观遗传修饰而实现的.

科学领域:

  • 生殖生物学
  • 发育生物学
  • 灵长类研究

背景情况:

  • 体细胞核转移 (SCNT) 是克隆哺乳动物的一种技术.
  • 由于早期发育障碍,灵长类的SCNT效率仍然很低.
  • 对于成功的SCNT来说, 表观遗传重编程至关重要.

研究的目的:

  • 建立一个更有效的SCNT方法.
  • 克服SCNT早期发展中的表观遗传障碍.
  • 为了生产健康的克隆灵长类动物.

主要方法:

  • 使用胎儿纤维细胞作为捐赠细胞.
  • 在卵细胞中人工增强的表观遗传修饰剂.
  • 在中进行体细胞核转移.

主要成果:

  • 他们成功地克隆了两只健康的子.
  • 证明了增强的SCNT技术的可行性.
  • 克服了SCNT的早期发展障碍.

结论:

  • 卵细胞的增强表观遗传修饰显著提高了灵长类动物的SCNT成功率.

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  • 这项研究为克隆灵长类动物提供了一种可行的方法.
  • 这些发现为灵长类繁殖技术的进一步发展铺平了道路.