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An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice06:40

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

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This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal...
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A reference measurement procedure for the absolute quantification of Aβ1-42 in human CSF based on solid-phase extraction and liquid chromatography tandem mass spectrometry is...
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We present a biochemical and behavioral protocol to evaluate the efficacy of mitochondria-targeted water-soluble compounds for the treatment of Spinocerebellar ataxia type 1 (SCA1) and other cerebellar neurodegenerative diseases.
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Here, we describe a simple method of intracerebroventricular and intravascular injection of viral particles or fluorescent microbeads into the neonatal mouse brain. The localization pattern of the virus and nanoparticles could be detected by microscopic evaluation or by in situ hybridization.
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Ascorbate plays numerous important roles in cellular metabolism, many of which have only come to light in recent years. Here we describe a medium-throughput, specific and inexpensive microplate assay for the determination of both intra- and extracellular ascorbate in cell culture.
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相关实验视频

Updated: Jan 8, 2026

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
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An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Published on: March 19, 2018

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使用集成光子的光频合成器

Daryl T Spencer1, Tara Drake2, Travis C Briles2,3

  • 1Time and Frequency Division, National Institute of Standards and Technology, Boulder, CO, USA. daryl.spencer@nist.gov.

Nature
|April 27, 2018
PubMed
概括
此摘要是机器生成的。

我们使用集成光子技术开发了一种紧的光频合成器. 这种设备精确控制光的频率, 使先进的光学工具更容易获得和负担得起.

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Last Updated: Jan 8, 2026

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科学领域:

  • 光子学
  • 量子光学
  • 测量学

背景情况:

  • 对于先进的科学来说, 光频合成器至关重要,
  • 综合光子学为复杂的光学系统提供了缩小和降低成本的途径.

研究的目的:

  • 使用集成光子技术开发一个紧,低成本,高效的光频合成器.
  • 在微芯片上展示相连贯的微波-光学链接.

主要方法:

  • 使用一个异质集成的III-V/可调节激光器.
  • 在芯片上制造的非线性频率.
  • 使用离芯片激光器实现了送.

主要成果:

  • 在1550nm附近以1Hz分辨率实现可编程激光频率输出.
  • 证明了异常的频率稳定性,合成误差为7.7 × 10−15.
  • 该系统利用集成光学来实现相连贯的微波-光学链接.

结论:

  • 这种集成光子学方法显著降低了光频合成器的尺寸,功率和成本.
  • 开发的技术使高精度光学频源在各种科学和计量应用中得到更广泛的应用.
  • 大量的半导体制造可以促进这些紧型设备的广泛使用.