用CODEX多重成像对老鼠骨结构进行深度分析
Yury Goltsev1, Nikolay Samusik1, Julia Kennedy-Darling1
1Department of Microbiology and Immunology, Baxter Laboratory in Stem Cell Biology, Stanford University School of Medicine, Stanford, CA, USA.
通过索引共检测 (CODEX) 揭示了免疫细胞社区如何影响健康和狼小鼠的蛋白质表达. 这种空间细胞计量方法揭示了疾病中的新型细胞相互作用.
科学领域:
- 免疫学
- 细胞生物学
- 生物技术
背景情况:
- 免疫细胞的相互作用对组织功能和疾病至关重要.
- 了解淋巴组织中的空间关系是复杂的.
- 多重成像为详细的细胞分析提供了潜力.
研究的目的:
- 通过索引应用协同检测 (CODEX) 进行大鼠脏的高复合空间分析.
- 研究细胞邻居对免疫细胞蛋白质表达的影响.
- 在健康与自身免疫疾病模型中比较脏结构和细胞相互作用.
主要方法:
- 通过索引利用联合检测 (CODEX),一种代抗体可视化技术.
- 使用DNA条形码,光核酸和现场聚合以进行索引.
- 在密集组织中开发了一种单细胞抗原量化算法.
主要成果:
- 观察到细胞邻居对免疫细胞受体表达的显著影响.
- 在MRL/ lpr狼小鼠中描述了以前未知的细胞相互作用动态.
- 在单细胞和邻居水平上进行了新的淋巴组织结构分析.
结论:
- CODEX提供高准确度多重空间细胞计量用于定量组织分析.
- 细胞邻居环境对免疫细胞的表型有很大影响.
- 这种方法揭示了自身免疫性疾病中明显的脏细胞动态.
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