控制直角核糖体子单元的相互作用使新的功能进化成为可能
Wolfgang H Schmied1, Zakir Tnimov1, Chayasith Uttamapinant1,2
1Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
Nature
|December 7, 2018
概括
工程设计
科学领域:
- 合成生物学
- 分子生物学
- 生物化学
背景情况:
- 正角核糖体是为了特定的mRNA转化而设计的核糖体.
- 现有的直角核糖体与内生核糖体共享子单元,限制了功能进化.
- 控制大子单元的结合是进化新核糖体功能的关键.
研究的目的:
- 设计具有受控子单位关联的新型直角核糖体.
- 为了使直角核糖体中的大子单元功能进化.
- 建立单独依赖于 ribosome 的细胞系统.
主要方法:
- 通过连接16S和23S核糖体RNA来设计和制造"串联"的正交核糖体.
- 作为唯一的转化机制, 进化细胞具有基因组编码的结合核糖体.
- 使用冷电子显微镜确定工程核糖体的结构.
- 通过创建聚合新型单体序列的核糖体来证明功能进化.
主要成果:
- 工程结合的核糖体具有高活性,与父直角核糖体相美.
- 嵌入式核糖体将与内源性核糖体子单元的关联最小化.
- 作为唯一的核糖体支持生长的细胞.
- 低温电磁可视化显示了主体介导子单位的结构基础.
- 进化接的核糖体有效地转换了自然核糖体无法转换的序列.
结论:
- 工程结合的核糖体提供了对子单元的关联和功能的精确控制.
- 这种系统使整个直角核糖体的进化成为新的生物合成.
- 提供了在细胞内创建非正规生物聚合物的基础.
相关概念视频
Ribosomes
75.7K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
75.7K
Ribosomes
10.9K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
10.9K
Ribosomal RNA Synthesis
14.8K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.8K
Orthogonal Trajectories
61
Orthogonal trajectories describe the geometric relationship between two families of curves that intersect each other at right angles. One illustrative case involves a family of parabolas that open sideways along the x-axis. These curves share a common shape but differ by a scaling parameter, resulting in a set of curves that all pass through the origin and widen at different rates.Determining Orthogonal TrajectoriesTo identify the orthogonal trajectories for these parabolas, the first step...
61
Convergent Evolution
32.9K
Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
32.9K
The Evidence for Evolution
48.1K
Genetic variations accumulating within populations over generations give rise to biological evolution. Evolutionary changes can result in the formation of novel varieties and entire new species. These changes are responsible for the diverse forms of life inhabiting the planet. The evidence for evolution suggests that all living organisms descended from common ancestors.
48.1K


