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相关概念视频

Antibody Structure01:10

Antibody Structure

65.7K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
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Conformity01:20

Conformity

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Conformity is the change in a person’s behavior to go along with the group, even if that person does not agree with the group.
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Antibody Structure and Classes01:25

Antibody Structure and Classes

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Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
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Conformations of Butane02:20

Conformations of Butane

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Unlike ethane and propane that have only two major conformations, butane has more than two conformers. The staggered form of butane in which the bulky methyl groups on the two carbons are placed on opposite sides, that is, at a dihedral angle of 180°, is the lowest energy, most stable form — called the anti conformer. This conformation is stabilized due to the absence of steric repulsion between the largely spaced out methyl groups. The other two staggered conformations are...
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Trial and Error and Algorithm01:12

Trial and Error and Algorithm

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A problem-solving strategy is a plan of action used to find a solution. Different strategies have distinct action plans. Trial and error involves trying different solutions until one works. For instance, to fix a broken printer, you might check ink levels, ensure the paper tray isn't jammed, and verify the printer's connection to your laptop. This method can be time-consuming but is commonly used. Thomas Edison, for example, used trial and error to find a suitable filament for the light...
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Conformations of Cycloalkanes02:29

Conformations of Cycloalkanes

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Adolf von Baeyer attempted to explain the instabilities of small and large cycloalkane rings using the concept of angle strain — the strain caused by the deviation of bond angles from the ideal 109.5° tetrahedral value for sp3  hybridized carbons. However, while cyclopropane and cyclobutane are strained, as expected from their highly compressed bond angles, cyclopentane is more strained than predicted, and cyclohexane is virtually strain-free. Hence, Baeyer’s theory that...
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相关实验视频

Updated: Feb 9, 2026

Author Spotlight: Exploring Behavioral Pathways Through Cross-Species Insights in Foraging and Communication
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Author Spotlight: Exploring Behavioral Pathways Through Cross-Species Insights in Foraging and Communication

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抗体超变循环的结构,由一个 conformational 搜索算法再现.

R E Bruccoleri1, E Haber, J Novotný

  • 1Cellular and Molecular Research Laboratory, Massachusetts General Hospital, Boston 02114.

Nature
|October 6, 1988
PubMed
概括

研究人员使用计算方法成功建模了抗体超变循环. 这种技术准确地重建了抗原组合部位,推进了抗体工程和蛋白质结构预测.

科学领域:

  • 结构生物学 结构生物学
  • 免疫学 免疫学 免疫学
  • 计算化学的计算化学

背景情况:

  • 抗体特异性是由抗原组合部位内的超变循环决定的.
  • 这些不规则循环的准确建模对于蛋白质结构预测具有挑战性.
  • 了解循环结构对于抗体工程和药物设计至关重要.

研究的目的:

  • 在抗体分子中计算重建删除的超变循环.
  • 用已知的抗体晶体结构验证一个建模协议.
  • 评估从序列中预测完整的抗原组合部位的可行性.

主要方法:

  • 从小鼠免疫球蛋白的X射线结构中删除超变循环.
  • 使用构造搜索程序CONGEN.使用循环的重建.
  • 生成循环形状和组合现场模型的构建.

主要成果:

  • 在McPC 603抗体中开发了一个成功的超变循环重建协议.
  • 模拟HyHEL-5抗体中的超变循环,与晶体结构达成良好一致.
  • 证明了对抗体组合部位的计算建模的准确性.

结论:

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Assessment of Immunologically Relevant Dynamic Tertiary Structural Features of the HIV-1 V3 Loop Crown R2 Sequence by ab initio Folding
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  • 计算机建模可以准确地重建抗体超变循环.
  • 开发的协议显示了预测完整的抗原组合部位的前景.
  • 这项工作促进了对抗体设计和蛋白质结构预测的未来研究.