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相关概念视频

Next-generation Sequencing03:00

Next-generation Sequencing

93.2K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Genomics02:02

Genomics

37.8K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Sanger Sequencing01:57

Sanger Sequencing

759.5K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
759.5K
Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

4.3K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
4.3K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

19.4K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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相关实验视频

Updated: Sep 28, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

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一个下一代人类基因组序列

Deanna M Church1

  • 1Inscripta, Inc., Boulder, CO, USA.

Science (New York, N.Y.)
|March 31, 2022
PubMed
概括
此摘要是机器生成的。

几乎完整的人类基因组序列提供了一个新的参考,为更全面的遗传研究和理解铺平了道路. 这一进步旨在改善基因组研究中的代表性.

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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

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相关实验视频

Last Updated: Sep 28, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

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科学领域:

  • 基因组学
  • 生物信息学
  • 人口遗传学

背景情况:

  • 人类基因组参考序列对于遗传研究至关重要.
  • 目前的参考基因组缺乏多样性,可能会影响研究结果.
  • 需要一个更具包容性和代表性的人类基因组参考.

研究的目的:

  • 呈现一个几乎完整的人类基因组序列.
  • 建立一个更包容的基因组参考的基础.
  • 在人类遗传研究中促进更广泛的代表性.

主要方法:

  • 使用先进的测序技术.
  • 使用生物信息管道进行序列组装和分析.
  • 使用比较基因组学方法来评估包容性.

主要成果:

  • 一个高质量的,几乎完整的基因组序列被生成.
  • 新的序列包含了以前从参考基因组中缺失的区域.
  • 初步分析显示,不同群体的代表性有所提高.

结论:

  • 开发的基因组序列代表了向更具包容性的人类参考迈出的重要一步.
  • 这种资源有可能减少基因研究中的偏见.
  • 需要进一步努力才能充分实现具有普遍代表性的基因组参考.