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Updated: Sep 1, 2025

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography
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通过三维电子衍射在框架材料中直接定位有机分子

Meng Ge1, Taimin Yang1, Hongyi Xu1

  • 1Department of Materials and Environmental Chemistry, Stockholm University, Stockholm SE-106 91, Sweden.

Journal of the American Chemical Society
|August 11, 2022
PubMed
概括

三维电子衍射 (3D ED) 现在可以在纳米晶体框架内初步确定客分子的结构. 这种强大的技术能够高精度地揭示宿主与客人的交互,

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X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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