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一种能够复制的人类腺病毒55型编码EGFP的生成和表征.

Wei Li1, Yuehong Chen1, Ye Feng1

  • 1State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.

Viruses
|May 27, 2023
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概括

一种新的传染性人类腺病毒55 (HAdV-55) 病毒,rAdv55-dE3-EGFP,被创建用于快速抗病毒查. 该工具有助于量化中和抗体,并开发急性呼吸道疾病 (ARD) 的新疗法.

关键词:
加强了GFP的支持.同类的重组组合.人类腺病毒55型传染性的克隆病毒.具有复制能力的复制能力.病毒报告员病毒报告员

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科学领域:

  • 病毒学 病毒学
  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学

背景情况:

  • 人类腺病毒55 (HAdV-55) 爆发在平民和军人中引起严重的急性呼吸道疾病 (ARD).
  • 开发抗病毒抑制剂和量化中和抗体需要快速的病毒感染监测系统.
  • 从等离子体中产生传染性病毒对于创建实验监测系统至关重要.

研究的目的:

  • 构建HAdV-55的全长传染性cDNA克隆,用于生成复合病毒.
  • 开发用于HAdV-55中和测试和抗病毒查的快速,高通量测试.

主要方法:

  • 通过细菌介导的重组被用来创建HAdV-55基因组的pAd55-FL克隆.
  • 一个绿色光蛋白 (GFP) 表达盒取代了E3区域,创建了pAd55-dE3-EGFP.
  • 重组病毒rAdv55-dE3-EGFP被救出并以稳定性和复制性为特征.

主要成果:

  • 重组病毒rAdv55-dE3-EGFP是基因稳定的,在细胞培养中与野生型HAdV-55类似地复制.
  • rAdv55-dE3-EGFP有效量化了血清中的中和抗体,与传统的微中和试验保持一致.
  • 该试验证明了使用A549细胞进行抗病毒查的实用性.

结论:

  • rAdv55-dE3-EGFP病毒是快速HAdV-55中和测试的可靠工具.
  • 这种高通量试验可促进对HAdV-55.5的有效抗病毒查.
  • 开发的系统支持针对HAdV-55诱导的ARD的对策的开发.