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第一个报告的第一份报告.

Marcel Wenneker1, Khanh Pham2,3, Kiki Kots4

  • 1Wageningen University and Research Centre (WUR), Field Crops, Zetten, Netherlands; marcel.wenneker@wur.nl.

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概括
此摘要是机器生成的。

这项研究确定了Didymella macrostoma是荷兰人尾腐烂的原因.

关键词:
一个因果代理.农作物类型 农作物类型病理起因 病理起因 病理起因果实 果实是一种水果.菌的真菌是一种课题区域 课题区域收益损失和经济影响.树上的果实 树上的果实 树上的果实

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科学领域:

  • 植物病理学 植物病理学
  • 菌类学 菌类学是指菌类学.
  • 园艺园艺 园艺园艺

背景情况:

  • 荷兰的梨 ("会议") 生产面临着收获后的损失,原因是末腐烂.
  • 在2019-2021年期间,在荷兰包装工厂的"会议"梨上观察到1%至5%的末腐烂.
  • 症状包括 1-3 厘米的棕色死,围绕梨子的葡萄杯末端.

研究的目的:

  • 为了隔离和识别导致荷兰"会议"梨的末腐烂的真菌病原体.
  • 用科赫的假设来确认孤立的真菌的致病性.
  • 为开发对抗这种梨子收获后疾病的控制策略提供基础知识.

主要方法:

  • 从受感染的梨病变中将真菌分离到土豆糖 (PDA) 上.
  • 在PDA和麦粉 agar (OA) 上的真菌殖民地和 conidia 的形态特征.
  • 多部位基因测序 (ITS,LSU,TUB,TEF1) 用于精确的真菌鉴定.
  • 对"会议"梨进行致病性测试,以满足科赫的假设.

主要成果:

  • 一种在形态上与Didymella macrostoma (同义词) 一致的真菌. 瘤巨瘤) 被分离出来.
  • 基因测序证实了四个分离物是Didymella macrostoma,与GenBank记录的高序列相同.
  • 科赫的假设得到了成功的完成,将D. macrostoma从人工注射的梨中重新分离出来,这些梨表现出典型的尾腐烂症状.

结论:

  • 迪迪梅拉 (Didymella macrostoma) 被确定为荷兰梨子中尾腐烂的致病原体.
  • 这是首次报告D. macrostoma导致梨中的末腐烂.
  • 准确识别病原体对于有效管理收获后梨病至关重要.