Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Ligand Binding Sites02:40

Ligand Binding Sites

12.9K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
12.9K
Protein-protein Interfaces02:04

Protein-protein Interfaces

12.6K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.6K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.8K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.8K
Conserved Binding Sites01:49

Conserved Binding Sites

4.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.2K
Protein Networks02:26

Protein Networks

4.0K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Eyes Toward the Clinic: Selective Inhibition and Degradation Approaches to Bromodomain-Containing Proteins.

Chembiochem : a European journal of chemical biology·2026
Same author

Development of a Lysine-Reactive Targeted Covalent Inhibitor for the P300/CBP-Associated Factor Bromodomain Through Structure-Based Design.

ChemMedChem·2026
Same author

Structural Basis for BD1-Preferring 2,4-Disubstituted Pyrimidine BRDT Inhibitors.

Journal of medicinal chemistry·2026
Same author

NanoBRET Tracer Development for Class I Bromodomain Target Engagement in Live Cells.

Bioconjugate chemistry·2026
Same author

NanoBRET Tracer Development for Class I Bromodomain Target Engagement in Live Cells.

bioRxiv : the preprint server for biology·2025
Same author

Call for Papers: Bridging Gaps in Global Health with Medicinal Chemistry.

Journal of medicinal chemistry·2025

相关实验视频

Updated: Jul 27, 2025

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.1K

使用蛋白质观测的碎片基联体发现.

Scott K Bur1, William C K Pomerantz2, Morgan L Bade1

  • 1Department of Chemistry, Gustavus Adolphus College, St. Peter, Minnesota 56028, United States.

Journal of chemical education
|June 5, 2023
PubMed
概括
此摘要是机器生成的。

本研究介绍了一个为期10周的基于课程的本科研究经验 (CURE) 有机化学实验室. 学生使用蛋白质观察的19F核磁共振来发现小分子配体,增强STEM保留.

关键词:
基于调查/发现的学习学习.实验室说明书 实验室说明书核磁共振光谱法 核磁共振光谱法有机化学 有机化学蛋白质 / 类蛋白质

更多相关视频

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.9K
Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA
10:21

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA

Published on: February 23, 2024

2.7K

相关实验视频

Last Updated: Jul 27, 2025

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.1K
Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.9K
Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA
10:21

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA

Published on: February 23, 2024

2.7K

科学领域:

  • 有机化学 有机化学
  • 生物物理化学 生物物理化学
  • 化学教育 化学教育

背景情况:

  • 基于课程的本科研究经验 (CUREs) 在STEM教育中越来越多地被采用.
  • 在STEM学科中,CUREs已经证明了提高学生留学率的有效性.
  • 有机化学课程可以从整合研究经验中受益.

研究的目的:

  • 描述了一个为期10周的CURE,用于第二学期的有机化学实验室.
  • 将小分子合成与生物物理测量结合起来,使用蛋白观测的19F NMR (PrOF NMR).
  • 与传统实验室相比,评估学生的技能发展和自我感知的收益.

主要方法:

  • 学生们进行了小分子的多步有机合成.
  • 用蛋白观测的19F NMR (PrOF NMR) 来确定小分子与目标蛋白的结合亲和力 (Kd).
  • 结合了结构-活性关系 (SAR) 研究和定量生物物理测量.

主要成果:

  • 治疗成功地向学生介绍了有机合成,SAR和定量生物物理测量.
  • PrOF的NMR实验提供了对联体蛋白结合的快速评估.
  • 在CURE中,学生自我感知的技能增长超过了传统和基于调查的实验室.

结论:

  • 使用 PrOF NMR 的基于碎片的配体发现实验室在本科有机化学中很容易实现.
  • 这个CURE提高了生物物理测量的科学素养和实践技能.
  • CURE模型对提高学生在化学中的参与度和留学能力充满希望.