使用蛋白质观测的碎片基联体发现
Scott K Bur1, William C K Pomerantz2, Morgan L Bade1
1Department of Chemistry, Gustavus Adolphus College, St. Peter, Minnesota 56028, United States.
Journal of chemical education
|June 5, 2023
概括
本研究介绍了一个为期10周的基于课程的本科研究经验 (CURE) 有机化学实验室. 学生使用蛋白质观察的19F核磁共振来发现小分子配体,增强STEM保留.
科学领域:
- 有机化学 有机化学
- 生物物理化学 生物物理化学
- 化学教育 化学教育
背景情况:
- 基于课程的本科研究经验 (CUREs) 在STEM教育中越来越多地被采用.
- 在STEM学科中,CUREs已经证明了提高学生留学率的有效性.
- 有机化学课程可以从整合研究经验中受益.
研究的目的:
- 描述了一个为期10周的CURE,用于第二学期的有机化学实验室.
- 将小分子合成与生物物理测量结合起来,使用蛋白观测的19F NMR (PrOF NMR).
- 与传统实验室相比,评估学生的技能发展和自我感知的收益.
主要方法:
- 学生们进行了小分子的多步有机合成.
- 用蛋白观测的19F NMR (PrOF NMR) 来确定小分子与目标蛋白的结合亲和力 (Kd).
- 结合了结构-活性关系 (SAR) 研究和定量生物物理测量.
主要成果:
- 治疗成功地向学生介绍了有机合成,SAR和定量生物物理测量.
- PrOF的NMR实验提供了对联体蛋白结合的快速评估.
- 在CURE中,学生自我感知的技能增长超过了传统和基于调查的实验室.
结论:
- 使用 PrOF NMR 的基于碎片的配体发现实验室在本科有机化学中很容易实现.
- 这个CURE提高了生物物理测量的科学素养和实践技能.
- CURE模型对提高学生在化学中的参与度和留学能力充满希望.
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