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Updated: Jun 15, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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协调越来越多的化RNA吸收器工具箱用于RNA检测和成像.

Xiaocen Lu1, Kristen Y S Kong1, Peter J Unrau1

  • 1Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada. punrau@sfu.ca.

Chemical Society reviews
|June 6, 2023
PubMed
概括

化RNA的阿帕特马为RNA检测和成像提供明亮,可调的光,克服了自然光分子的局限性. 本综述详细介绍了它们的选择,评估和应用在先进的生物成像中.

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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