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相关概念视频

Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

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Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
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Non-gated Ion Channels01:24

Non-gated Ion Channels

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Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
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Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

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Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
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Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.2K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
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Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

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Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
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Ion Channels01:19

Ion Channels

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The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
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相关实验视频

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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

771

双孔通道 1 和 Ca 的通道.

Enrique I Oliver1,2, Martina Jabloñski3, Mariano G Buffone3

  • 1Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Autónoma de México, Cuernavaca, Morelos, México.

The Journal of physiology
|June 6, 2023
PubMed
概括
此摘要是机器生成的。

精子功能至关重要的体化,通过TPC1和CRAC道触发的释放. 这项研究揭示了控制信号传递的关键通路,在小鼠精子中的体反应 (AR) 期间.

关键词:
在 CRAC 频道上播放CRAC频道.在Ca2+信号中.在TPC1频道.性化是一种性性化.这是一个宏观的反应反应.一个体囊泡.

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One-channel Cell-attached Patch-clamp Recording
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Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
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Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms

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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

771
One-channel Cell-attached Patch-clamp Recording
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One-channel Cell-attached Patch-clamp Recording

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Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
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科学领域:

  • 生殖生物学 生殖生物学
  • 细胞生物学 细胞生物学
  • 生物化学 生物化学

背景情况:

  • 宏体反应 (AR) 对于哺乳动物受精至关重要,涉及 (Ca2+) 和宏体化.
  • 在AR过程中Ca2+运输的精确分子机制,特别是与pH值变化相关的,仍然不清楚.

研究的目的:

  • 为了研究介导Ca2+信号的途径,由鼠标精子的体pH (pHa) 增加引起的Ca2+信号.
  • 阐明TPC1和CRAC通道在调节体化过程中的Ca2+流量的作用.

主要方法:

  • 在小鼠精子中单细胞Ca2+成像.
  • 药理上抑制精子特定的Ca2+通道 (CatSper) 和双孔通道 (TPC1).
  • 使用 lysosomotropic 剂 Gly-Phe-β-naphthylamide (GPN) 来评估透贡献.

主要成果:

  • 米贝弗拉迪尔和NNC 55-0396诱导了pHa的增加和体内Ca2+的释放,而不会损害体内膜.
  • 通过TPC1通道的抑制减少了Ca2+的流入,这种流入是由性化刺激的.
  • CRAC通道阻塞减少了Ca2+吸收,由pHa升高引发.

结论:

  • 宏性化触发了Ca2+的释放和通过TPC1和CRAC道的吸收.
  • 这些发现澄清了pHa在控制Ca2+在体反应期间的流出和流入中的作用.
  • 这项研究提高了对精子受精的分子基础的理解.