益生菌衍生P8蛋白在结肠直肠癌细胞系DLD-1中的抗癌作用
Byung Chull An1, Jun Young Ahn1, Daebeom Kwon1
1R&D Center, Cell Biotech, Co., Ltd., 50 Aegibong-ro 409 Beon-gil, Gaegok-ri, Wolgot-myeon, Gimpo-si 10003, Gyeonggi-do, Republic of Korea.
International journal of molecular sciences
|June 28, 2023
概括
益生菌中的一种新型蛋白质P8,通过破坏细胞循环调节和Wnt信号通路,阻止结直肠癌 (CRC) 的生长. 这一发现为CRC治疗提供了新的治疗途径.
科学领域:
- 分子生物学分子生物学
- 癌症研究 癌症研究
- 微生物学 微生物学
背景情况:
- 结肠直肠癌 (CRC) 仍然是一个重大的健康挑战.
- 益生菌衍生化合物在癌症治疗中显示出潜力.
- 对于P8抗癌作用的确切机制尚未完全理解.
研究的目的:
- 阐明P8抑制结直肠癌 (CRC) 细胞生长的分子机制.
- 确定参与P8细胞吸收和下游目标的蛋白质.
- 研究P8对细胞循环调节和CRC中的Wnt信号的影响.
主要方法:
- 使用P8作为诱来识别DLD-1细胞溶解物中的相互作用蛋白质的拉下测试.
- 分析P8在细胞质和细胞核中的定位和相互作用.
- 评估P8对GSK3β活性,β-catenin降解和基因转录的影响.
主要成果:
- 确定了KPNA3和GSK3β作为P8相互作用蛋白.
- P8 防止了细胞质中GSK3β的无活化,导致β-catenin的降解.
- 通过KPNA3,P8转移到核中,在那里它破坏了GSK3β基因转录的调节.
结论:
- P8通过抑制GSK3β和降低Wnt信号调节来抑制CRC生长.
- 即使在Wnt激活的条件下,P8也会诱导CRC细胞的细胞周期停止.
- P8代表着对结直肠癌的一种有前途的治疗药物.
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