一个体外小肠模型,包含食物矩阵和细菌模拟社区,用于检测肠功能.
Mridu Malik1,2, Jacob V Tanzman2,3, Sanat Kumar Dash1,2
1Department of Biomedical Engineering, Binghamton University, Binghamton, NY 13902, USA.
Microorganisms
|June 28, 2023
概括
食品添加剂二氧化纳米颗粒 (TiO2NP) 没有影响肠道透性,但增加了甘油三的运输. 细菌群落的存在逆转了这种效应,并增强了葡萄糖的运输.
科学领域:
- 胃肠病学 胃肠病学
- 纳米技术纳米技术
- 微生物学 微生物学
背景情况:
- 小肠是饮食和微生物群之间的复杂相互作用的所在.
- 像二氧化纳米颗粒 (TiO2 NPs) 这样的食品添加剂很常见,需要了解它们的生物影响.
- 现有的模型往往缺乏足够的复杂性,无法完全代表体内条件.
研究的目的:
- 开发和利用一个复杂的体外模型的人类小肠.
- 研究TiO2NP对肠道上皮功能和营养物质运输的影响.
- 探索代表性细菌群落对这些相互作用的影响.
主要方法:
- 使用人类细胞,模拟消化和定义的细菌群体 (大肠杆菌,L. rhamnosus,S. salivarius,B. bifidum,E. faecalis) 建立一个复杂的体外小肠模型.
- 模型暴露于TiO2NP的生理学相关度.
- 评估上皮质透性,性酸酶活性,甘油三运输和葡萄糖运输.
- 评估细菌被困在粘液层中的情况.
主要成果:
- TiO2 NPs没有改变肠道的透性,但在没有细菌的情况下增加了甘油三的运输.
- 细菌群体的存在逆转了TiO2 NP诱导的甘油三运输的增加.
- 虽然单个细菌物种没有影响,但细菌群体增强了葡萄糖的运输.
- 暴露于TiO2NP导致细菌在粘液层中的捕获减少,可能是由于粘液厚度降低.
结论:
- 开发的体外模型有效模拟了小肠功能,包括宿主微生物群相互作用.
- 相关度的TiO2NP不会损害肠道屏障的完整性,但可以影响营养吸收的动态.
- 细菌群体在调节食品添加剂对营养物质运输的影响方面发挥着至关重要的作用,并且可能表现出特定于社区的行为.
- 该模型为研究营养成分和纳米颗粒对小肠生理和肠道微生物群的影响提供了一个有价值的平台.
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