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The Eukaryotic Promoter Region02:40

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The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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相关实验视频

Updated: Jul 23, 2025

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
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补充的Alu序列调解了增强剂-促进剂的选择性

Liang Liang1, Changchang Cao1, Lei Ji1

  • 1Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.

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概括
此摘要是机器生成的。

通过互补的RNA相互作用调解增强剂-促进剂循环. 这一发现揭示了基因调节的机制, 并将非编码变体与分子功能联系起来, 包括癌症风险.

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科学领域:

  • 基因组学
  • 分子生物学
  • 表观遗传学

背景情况:

  • 增强剂通过与促进体相互作用来调节基因表达.
  • 增强剂找到它们的相关促进剂的机制在很大程度上是未知的.

研究的目的:

  • 研究RNA相互作用在增强剂-促进剂连接中的作用.
  • 绘制增强剂-促进剂RNA相互作用并识别相关的基因组元素.
  • 将非编码的遗传变体与它们的分子功能联系起来.

主要方法:

  • 应用RICS技术来生成增强剂-促进剂RNA相互作用图.
  • 分析Alu元素与增强剂-促进剂RNA相互作用位点的重叠.
  • 功能性实验涉及Alu元素的淘汰和绑定.
  • 将非编码风险变体映射到增强剂-促进剂相互作用图中.

主要成果:

  • 37. 9% 的增强剂-促进剂RNA相互作用部位与 Alu 序列重叠.
  • 阿卢和非阿卢RNA序列显示互补性,表明双重形成.
  • 元素操纵 (淘汰赛,插入,绑定) 影响了增强剂-促进剂循环.
  • 建立了一个框架,将非编码变体与基因功能联系起来,识别了影响蛋白质编码基因的Alu元素中的数千种变体.
  • 在PTK2增强剂中插入多态Alu与瘤发生有关.

结论:

  • 通过RNA双重组的形成来调解增强剂-促进剂配对的特异性,Alu元素起着至关重要的作用.
  • 这项研究提供了一种方法来解释非编码风险变体的分子功能.
  • 这些发现为基因调节及其对疾病的影响提供了新的理解.