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相关概念视频

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
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甲基化调节的长非编码RNA表达在性结肠炎.

Christopher G Fenton1,2, Mithlesh Kumar Ray1, Wei Meng1

  • 1Clinical Bioinformatics Research Group, Department of Clinical Medicine, UiT-The Arctic University of Norway, N-9037 Tromsø, Norway.

International journal of molecular sciences
|July 14, 2023
PubMed
概括

这项研究探讨了DNA甲基化与性结肠炎 (UC) 中长非编码RNA (lncRNA) 表达之间的联系. 它识别了特定的lncRNA和参与炎症的基因,为UC病变发生提供了新的见解.

关键词:
通过DNA甲基化.表观遗传学是指表观遗传学.长长的非编码RNAs.性结肠炎是一种

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科学领域:

  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 分子生物学分子生物学
  • 胃肠病学 胃肠病学

背景情况:

  • 长非编码RNAs (lncRNAs) 涉及到性结肠炎 (UC) 的发生.
  • 在UC中,DNA甲基化和lncRNA表达之间的相互作用仍未得到充分研究.
  • 环境因素和表观遗传修饰被认为有助于UC的发展.

研究的目的:

  • 研究UC中DNA甲基化和lncRNA表达之间的关系.
  • 在UC中通过差异甲基化区域 (DMR) 调节的特定lncRNAs的识别.
  • 为了发现潜在的下游基因受DMR调节的IncRNAs在UC的影响.

主要方法:

  • 分析全基因组双硫酸盐测序 (WGBS) 和来自UC患者和对照患者的粘膜活检的lncRNA表达数据.
  • 相关性分析以确定可能由上游DMRs调节的lncRNAs.
  • 确定与DMR调节的lncRNAs相关的近端蛋白质编码基因.

主要成果:

  • 已确定UC相关的lncRNAs,包括MIR4435-2HG,ZFAS1,IL6-AS1和Pvt1,可能受到DMRs的调节.
  • 发现了参与炎症免疫反应的下游基因,如SERPINB1,CCL18和SLC15A4,与DMR调节的lncRNAs相关.
  • 建立了DNA甲基化驱动的lncRNA调节和UC病原体之间的潜在联系.

结论:

  • DNA甲基化和lncRNA表达之间的相互作用是UC的重要因素.
  • 特定的lncRNA及其下游点可能在UC相关的炎症中发挥关键作用.
  • 对这些表观遗传相互作用的进一步研究可以提高对UC的理解和治疗.