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相关概念视频

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
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Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Forced Transdifferentiation01:28

Forced Transdifferentiation

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Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
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相关实验视频

Updated: Jul 22, 2025

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
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用不同的方法重新编程的人类8细胞类细胞之间的转录组差异.

Masahito Yoshihara1, Juha Kere2

  • 1Department of Biosciences and Nutrition, Karolinska Institutet, Stockholm, Sweden; Institute for Advanced Academic Research, Chiba University, Chiba, Japan; Department of Artificial Intelligence Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan.

Stem cell reports
|July 21, 2023
PubMed
概括
此摘要是机器生成的。

人类胚胎基因组激活 (EGA) 现在通过使用新型的人类8细胞样细胞 (8CLCs) 更好地理解. 这些模型来自多能干细胞,模仿早期发育,用于研究人类胚胎发生.

关键词:
八个细胞类细胞的细胞.8CLCs是什么意思布拉斯托梅尔分子是什么?胚胎基因组激活活动胚胎干细胞是一种胚胎干细胞.人类胚胎人类胚胎重编程是重新编程.翻译学 翻译学 翻译学 翻译学

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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
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相关实验视频

Last Updated: Jul 22, 2025

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科学领域:

  • 发展生物学 发展生物学
  • 干细胞生物学 干细胞生物学
  • 基因组学就是基因组学.

背景情况:

  • 胚胎基因组激活 (EGA) 对于发育至关重要,但人类EGA的理解很少.
  • 现有的小鼠模型并不完全代表人类早期发育.

研究的目的:

  • 要总结生成人类8细胞类细胞 (8CLCs) 的方法.
  • 为了比较8CLCs的转录组形状与人类胚胎发育.
  • 促进对人类EGA和胚胎生成的研究.

主要方法:

  • 从多能干细胞生成人类8细胞类细胞 (8CLCs).
  • 单细胞RNA测序 (scRNA-seq) 用于细胞身份.
  • 整合8CLC转录组数据与人类胚胎scRNA-seq数据集.

主要成果:

  • 五个独立的小组已经开发了生产8CLC的方法.
  • 8CLCs的转录组概况与早期人类胚胎有相似之处.
  • 对比分析验证了8CLC作为人类EGA的模型.

结论:

  • 人类8CLC为研究EGA提供了有价值的体外模型.
  • 这些模型可以详细描述人类植入前发育中的基因.
  • 使用8CLC进行进一步的研究将有助于更好地理解人类胚胎发生.