肌衍生矩阵交联技术用于电多层支架
Thomas L Jenkins1, Paula A Sarmiento Huertas1, Kentaro Umemori1
1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.
Journal of biomedical materials research. Part A
|July 25, 2023
概括
使用紫外线或EDC方法将肌衍生基质 (TDM) 交叉连接到聚-ε-caprolactone (PCL) 支架,可以增强细胞附着和肌状基质合成. 这一策略改善了TDM在支架上的保留,以更好地修复肌.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 肌撕裂往往导致不完全愈合和纤维化,需要改进修复策略.
- 组织工程方法利用细胞播种的支架与仿生因素来增强肌的修复.
- 在脚手架上有效保留仿生因素对于最大限度地提高治疗效益和最大限度地降低风险至关重要.
研究的目的:
- 评估交叉连接方法,以增强肌衍生矩阵 (TDM) 在电聚 (ε-caprolactone) (PCL) 支架上的保留.
- 评估交叉链接对人类脂肪干细胞 (hASC) 行为和肌状矩阵合成的影响.
主要方法:
- 在使用TDM的PCL薄膜上测试了紫外线 (UV) 和碳二胺 (EDC:NHS:COOH) 交叉链接方法.
- 评估了交联支架的TDM保留,hASC扩散和机械性能 (模块,刚性).
- 评估hASC诱导的状分化,并使用福里埃变换红外光谱分析支架组成.
主要成果:
- 没有经过测试的交叉链接方法显著保留了比单独涂层更多的TDM,但UV和EDC方法改善了hASC的传播.
- 与未经处理或TDM涂层的PCL脚手架相比,紫外线交联的脚手架表现出较高的模量和刚度.
- hASCs在紫外线交叉连接的支架上表现出增强的扩散,交叉连接没有改变特征性肌矩阵峰值.
结论:
- 交叉连接TDM到电PCL支架可以改善状矩阵合成.
- 紫外线或EDC交叉链接提供了一种可行的策略,用于增强PCL支架上的TDM保留.
- 这种方法有望改善肌组织工程和修复的结果.
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