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相关概念视频

Exocytosis00:50

Exocytosis

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Exocytosis is a process that releases molecules outside the cell. Like other bulk transport mechanisms, exocytosis requires energy.
Exocytosis is the opposite of endocytosis, which brings molecules inside the cell. Sometimes, the released materials are signaling molecules. For example, neurons typically use exocytosis to release neurotransmitters. Cells also use exocytosis to insert proteins such as ion channels into their cell membranes, secrete proteins for use in the extracellular matrix, or...
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Excess Pressure Inside a Drop and a Bubble01:13

Excess Pressure Inside a Drop and a Bubble

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The shape of a small drop of liquid can be considered spherical, neglecting the effect of gravity. This drop can further be considered as two equal hemispherical drops put together due to surface tension. The forces acting on the spherical drop are due to the pressure of the liquid inside the drop, the pressure due to air outside the drop, and the force due to the surface tension acting on the two hemispherical drops.
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Exocrine Glands: Methods of Secretion01:08

Exocrine Glands: Methods of Secretion

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Exocrine glands are those that release their secretions through ducts. Based on their mode of secretion, they can be classified into merocrine, apocrine, and holocrine.
Merocrine Secretion
Merocrine secretion is the most common type of exocrine secretion. The secretions are enclosed in vesicles and moved to the cell's apical surface, where the contents are released by exocytosis. For example, mucous, a watery secretion rich in the glycoprotein mucin, is a merocrine secretion. The eccrine...
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Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

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Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
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Receptor-mediated Endocytosis01:20

Receptor-mediated Endocytosis

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Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
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Deindividuation00:57

Deindividuation

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Deindividuation is a form of social influence on an individual’s behavior such that the individual engages in unusual or non-normal behavior while in a group setting. Why? Because in these group settings, the individual no longer sees themselves as an individual anymore, disinhibiting their behavior and personal restraint.
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相关实验视频

Updated: Jul 20, 2025

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis
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Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

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为了突破或隐藏.

Seungha Hwang1, Jin Young Kang1

  • 1Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.

Structure (London, England : 1993)
|August 4, 2023
PubMed
概括

菌体兰巴达CII蛋白控制了化和 lysogenic 路径之间的切换. 这项研究揭示了CII依赖转录复合体的冷EM结构,解释了CII如何激活 lysogeny 的 PRE促进体.

科学领域:

  • 分子生物学分子生物学
  • 结构生物学 结构生物学
  • 病毒学 病毒学

背景情况:

  • 菌体lambda (λ) 感染涉及临界决定在化和 lysogenic 途径之间.
  • 菌体调节蛋白CII是这种途径选择的关键决定因素.
  • 了解CII介导的转录激活的分子机制对于破译病毒生命周期调节至关重要.

研究的目的:

  • 阐明由 λ 菌体 CII 蛋白激活转录的结构基础.
  • 揭示CII如何与PRE促进体相互作用以启动 lysogenic 途径.
  • 提供CII-依赖转录激活复合物的高分辨率结构模型.

主要方法:

  • 使用冷电子显微镜 (cryo-EM) 来确定该复合物的结构.
  • 结构分析和分子建模被用来解释复合体内的相互作用.
  • 生物化学测试可能被用来验证结构的功能影响 (推断).

主要成果:

  • 确定了在PRE促进体的λCII-依赖转录激活复合物的冷-EM结构.
  • 他们可视化了 λCII,促进体 DNA 和潜在宿主因子之间的详细原子相互作用.
  • 该结构揭示了 λCII 稳定预启动复合体以激活转录的精确机制.

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结论:

  • 确定的结构为 λCII 中介转录激活的分子机制提供了前所未有的洞察力.
  • 这种结构性理解阐明了菌体λ如何精确地控制切换到 lysogenic 途径.
  • 这些发现对理解其他病毒系统和 prokaryotes 中的转录调节有影响.