Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Stem Cell Culture01:17

Stem Cell Culture

5.2K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
5.2K
Embryonic Stem Cells00:57

Embryonic Stem Cells

3.6K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
3.6K
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

2.8K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
2.8K
Lineage Commitment01:21

Lineage Commitment

3.0K
Commitment is the  process whereby stem cells:
3.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

LASER couples damage sensing to ESCRT assembly for lysosome repair.

Nature·2026
Same author

Simultaneous capture of single cell RNA-seq, ATAC-seq, and CRISPR perturbation enables multiomic screens to identify gene regulatory relationships.

Cell reports methods·2025
Same author

<i>SAMHD1</i> knockout hiPSC model enables high lentiviral transduction efficiency in myeloid cell types.

Frontiers in genetics·2025
Same author

MorPhiC Consortium: towards functional characterization of all human genes.

Nature·2025
Same author

A multiomics approach reveals RNA dynamics promote cellular sensitivity to DNA hypomethylation.

Scientific reports·2024
Same author

Variants in tubule epithelial regulatory elements mediate most heritable differences in human kidney function.

Nature genetics·2024

相关实验视频

Updated: Jul 19, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.7K

干细胞模型中的功能基因组学:考虑和应用

Kaivalya Shevade1,2, Sailaja Peddada1,2, Karl Mader1,2

  • 1Laboratory for Genomics Research, San Francisco, CA, United States.

Frontiers in cell and developmental biology
|August 9, 2023
PubMed
概括
此摘要是机器生成的。

人类多能干细胞分化协议推进了疾病建模. 克里斯普尔-Cas9技术可以在诱导多能干细胞 (iPSC) 中进行精确的基因编辑,用于药物发现和机制阐明.

关键词:
通过CRISPR查进行查.发现药物的发现.功能性基因组学 功能性基因组学人类疾病 人类疾病诱导多能干细胞 (iPSC) 是一种诱导多能干细胞.从iPSC衍生的模型

更多相关视频

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.3K
Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
08:48

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Published on: February 17, 2016

9.3K

相关实验视频

Last Updated: Jul 19, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.7K
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.3K
Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
08:48

Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

Published on: February 17, 2016

9.3K

科学领域:

  • 干细胞生物学 干细胞生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • 在过去的二十年中,人类多能干细胞分化协议取得了重大进展,增强了细胞类型特异性和组织复杂性.
  • 从患者中产生诱导多能干细胞 (iPSC) 允许在相关的细胞环境中进行疾病建模,有助于研究疾病病因和进展.
  • 从iPSC衍生的模型越来越多地用于药物查,以确定新疗法和治疗点.

研究的目的:

  • 审查诱导多能干细胞 (iPSC) 疾病建模中的当前技术.
  • 探索基于CRISPR的功能基因组学屏幕,用于识别基因修饰剂和治疗点.
  • 讨论在各种应用和疾病领域实施这些技术的实际考虑.

主要方法:

  • 使用CRISPR-Cas9技术,包括CRISPR干扰 (CRISPRi) 和CRISPR激活 (CRISPRa),用于iPSC中的精确基因编辑.
  • 采用CRISPR淘汰,CRISPRi和CRISPRa屏幕来系统和公正地识别基因修饰剂.
  • 审查关于iPSC疾病模型和CRISPR屏幕的现有文献.

主要成果:

  • 包括CRISPRi和CRISPRa在内的CRISPR-Cas9技术,使先进的基因编辑能够超越单基因修改.
  • 在iPSC模型中的CRISPR屏幕已经确定了能够协同或对抗疾病突变的基因修饰剂.
  • 这些选导致了疾病机制和潜在的治疗点的识别.

结论:

  • 在iPSC分化和基于CRISPR的功能基因组学方面的进展加速了疾病机制的发现和药物开发.
  • 尽管存在技术挑战,但iPSC模型中的CRISPR屏幕为治疗策略提供了强大的洞察力.
  • 这些技术的整合对个性化医疗和发现新疗法具有重大前景.