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Updated: Jun 17, 2026

Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
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Preparation of Mouse Brain Tissue for Immunoelectron Microscopy

Published on: July 20, 2010

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为预嵌入免疫电子显微镜冷替代脑组织进行再水分

Janeth Pérez-Garza1, Emily Parrish-Mulliken1, Zachary Deane1

  • 1Department of Physiology and Neurobiology, University of Connecticut, 75 North Eagleville Rd. Unit 3156, Storrs, CT 06269-3156, USA.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|August 16, 2023
PubMed
概括

我们开发了一种用于电子显微镜 (EM) 体积重建的冷固定方法. 这种技术使敏感的分子标记能够实现,同时保留超结构,用于详细的脑电路分析.

关键词:
在EM体积重建中,EM体积重建.抗体标签的标记方法电子显微镜的电子显微镜结替代的替代方法高压冷方式的高压冷.神经解剖学的神经解剖学

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相关概念视频

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...