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Updated: Jul 18, 2025

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
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在无放大杂交试验中发现的microRNA-128-2-3p中的突变

Sofie Slott1, Cecilie Schiøth Krüger-Jensen1, Izabela Ferreira da Silva2,3,4

  • 1Department of Chemistry, Technical University of Denmark, Kgs Lyngby, Denmark.

PloS one
|August 22, 2023
PubMed
概括

这项研究提出了一种新方法来检测血中突变的microRNAs. 该测试确定了与炎症性结肠炎和结肠直肠癌相关的microRNA-128-2-3p中的特定突变.

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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相关概念视频

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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