通过光板镜像对照的中视镜斜平面显微镜
Stephan Daetwyler1, Bo-Jui Chang1, Bingying Chen1
1Lyda Hill Department for Bioinformatics, UT Southwestern Medical Center, 6000 Harry Hines BLVD, Dallas, TX 75390, USA.
bioRxiv : the preprint server for biology
|August 23, 2023
概括
我们开发了一种半镜斜平面显微镜 (OPM),用于高速的全生物体成像. 这种新的显微镜提供亚细胞分辨率,使整个生物体的生物过程的详细可视化.
科学领域:
- 生物学 生物学 生物学
- 显微镜的使用方法
- 生物物理学的生物物理.
背景情况:
- 了解整个生物体的生物过程对于心血管研究,神经科学和发育生物学等领域至关重要.
- 现有的成像技术经常面临速度,分辨率或视野的限制,对于整个生物体的研究.
研究的目的:
- 介绍一种新型的中视镜斜平面显微镜 (OPM),用于整个生物体的高速亚细胞分辨率成像.
- 展示OPM在复杂生物系统的体内和体内成像方面的能力.
主要方法:
- 研发了一种半镜斜平面显微镜 (OPM),采用微镜来提高轴分辨率和光学切割.
- 使用快速重定焦的光片扩展成像深度,同时保持轴分辨率.
- 采用低放大对象用于大视野,使大体积的中视镜成像成为可能.
主要成果:
- 在3.7×1.5×1mm3的体积上实现了中视镜成像,侧面分辨率为~2.3μm,轴向分辨率为~9.2μm.
- 在体内和体内对斑马鱼血管系统和内皮细胞核进行了高速度 (12 Hz 获取率) 的成像.
- 产生了全生物体血液流动动态的定量地图.
结论:
- 中视镜OPM能够以高速度和亚细胞分辨率进行前所未有的全生物成像.
- 这项技术为研究整个生物体中复杂,相互关联的生物过程提供了强大的工具.
- 在生物体层面上,OPM有助于对动态生物过程 (如血液流动) 的定量分析.
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