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Related Concept Videos

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Related Experiment Video

Updated: Jun 12, 2026

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks
08:32

Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks

Published on: January 26, 2024

Axially Swept Light-Sheet Microscopy using scattering and fluorescence contrast mechanisms.

Connor Baroody1, Jinlong Lin2, Stefan Wilhelm1

  • 1University of Oklahoma, Research Laboratory, Norman, OK, USA.

Proceedings of Spie--The International Society for Optical Engineering
|June 11, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a novel light-sheet microscopy technique combining axially swept illumination with scattering for rapid 3D imaging. This method achieves high-quality results comparable to traditional fluorescence microscopy, even for non-fluorescent samples.

Keywords:
Expansion microscopyLight-sheetMicroscopyPoint spread functionScattering

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Area of Science:

  • Biomedical imaging
  • Optical microscopy
  • Biophysics

Background:

  • Light-sheet fluorescence microscopy (LSFM) offers rapid, gentle volumetric imaging.
  • Extending LSFM to non-fluorescent contrast, like light-scattering, is desirable.
  • Coherent light-sheet illumination causes interference and object-variant point-spread functions in scattering media.

Purpose of the Study:

  • To develop a 3D imaging method combining light-sheet illumination with light-scattering.
  • To overcome challenges of interference and complex point-spread functions in scattering samples.
  • To achieve 3D imaging quality comparable to LSFM using non-fluorescent contrast.

Main Methods:

  • Axially swept illumination combined with sparse, strong scatterers.
  • Utilizing gold nanoparticles within expanded breast cancer spheroids.
  • Leveraging axial sweeping to record both fluorescence and scattered signals.

Main Results:

  • Achieved 3D imaging quality comparable to traditional LSFM.
  • Demonstrated successful imaging of expanded breast cancer spheroids.
  • Successfully recorded both fluorescence and scattered signals using the same setup.

Conclusions:

  • Axially swept illumination with scattering enables high-quality 3D imaging.
  • This technique expands LSFM capabilities to non-fluorescent contrast mechanisms.
  • The method is effective for biological samples like cancer spheroids.